Abstract |
Our research intended to investigate the roles of mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) in acute myocardial infarction (AMI) via delivery of microRNA (miR)-302d-3p. AMI mouse models were established. EVs isolated from MSCs with miR-302d-3p mimic were injected near the infarct area or co-cultured with hypoxic cardiomyocytes to evaluate their effects. The expression of NF-κB pathway-related genes and inflammatory factors was determined. AMI mice exhibited downregulated miR-302d-3p and elevated MD2 and BCL6 levels. BCL6 was negatively targeted by miR-302d-3p and could bind to MD2 promoter to upregulate MD2 expression. MSCs-EVs, MSCs-EVs carrying miR-302d-3p, or BCL6 or MD2 silencing inactivated the NF-κB pathway and alleviated infarcted area, myocardial fibrosis, inflammation, apoptosis, and cardiac dysfunction in AMI mice. Besides, MSCs-EVs, MSCs-EVs carrying miR-302d-3p, or BCL6 or MD2 silencing diminished viability and inflammation but augmented apoptosis of hypoxic cardiomyocytes. Conclusively, MSCs-EVs carrying miR-302d-3p repressed inflammation and cardiac remodeling after AMI via BCL6/MD2/NF-κB axis.
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Authors | Yuanyuan Liu, Rongchun Guan, Jizhou Yan, Yueping Zhu, Shiming Sun, Yan Qu |
Journal | Journal of cardiovascular translational research
(J Cardiovasc Transl Res)
Vol. 15
Issue 4
Pg. 754-771
(08 2022)
ISSN: 1937-5395 [Electronic] United States |
PMID | 35194734
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. |
Chemical References |
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Topics |
- Mice
- Animals
- Ventricular Remodeling
- NF-kappa B
(metabolism)
- MicroRNAs
(genetics, metabolism)
- Mesenchymal Stem Cells
(metabolism)
- Extracellular Vesicles
(metabolism)
- Myocardial Infarction
(genetics, metabolism)
- Inflammation
(genetics, metabolism)
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