LncRNA HOXD-AS1 promotes oral squamous cell carcinoma by sponging miR-203a-5p.

Abstract

OBJECTIVE:

In the present study, we aimed to explore lncRNA HOXD cluster antisense RNA 1 (HOXD-AS1) expression in oral squamous cell carcinoma (OSCC) tissues, its biological roles, and the underlying potential mechanisms in OSCC progression.

MATERIALS AND METHODS:

HOXD-AS1 expression in paired OSCC and non-tumor tissues from 60 OSCC patients was determined by RT-qPCR. The effects of HOXD-AS1 and miR-203a-5p overexpression on expression of Annexin A4, a validated target of miR-203a-5p, were analyzed by RT-qPCR and Western blot. The roles of HOXD-AS1, miR-203a-5p, and Annexin A4 in the invasion and migration of OSCC cells were analyzed by Transwell assays.

RESULTS:

HOXD-AS1 overexpression in OSCC predicted poor survival. HOXD-AS1 was predicted to interact with miR-203a-5p, but its expression was not significantly correlated with miR-203a-5p. HOXD-AS1 overexpression increased Annexin A4 expression, while miR-203a-5p overexpression decreased Annexin A4 expression in OSCC cells. Transwell assays showed that invasion and migration of OSCC cells were enhanced by HOXD-AS1 and Annexin A4 overexpression but were reduced by miR-203a-5p overexpression. In addition, miR-203a-5p overexpression suppressed the role of HOXD-AS1 in cell movement and Annexin A4 expression.

CONCLUSIONS:

HOXD-AS1 may upregulate Annexin A4 by sponging miR-203a-5p in OSCC to promote cancer cell invasion and migration.

• Authors: Yuchao Zhang, Feng Wang, Yang Yu
• Journal: Oral diseases (Oral Dis) Vol. 29 Issue 4 Pg. 1505-1512 (May 2023) ISSN: 1601-0825 [Electronic] Denmark
• PMID: 35152529 (Publication Type: Journal Article)
• Copyright: © 2022 The Authors. Oral Diseases published by Wiley Periodicals LLC.

Chemical References

• MicroRNAs
• RNA, Long Noncoding
• Annexin A4

Topics

• Humans
• MicroRNAs (genetics, metabolism)
• Carcinoma, Squamous Cell (genetics, metabolism)
• RNA, Long Noncoding (genetics)
• Squamous Cell Carcinoma of Head and Neck (genetics)
• Annexin A4 (genetics, metabolism, pharmacology)
• Mouth Neoplasms (genetics, metabolism)
• Cell Proliferation (genetics)
• Neoplasm Invasiveness (genetics)
• Cell Line, Tumor
• Cell Movement (genetics)
• Gene Expression Regulation, Neoplastic
• Head and Neck Neoplasms (genetics)