Pyridoxal phosphate, pyridoxamine phosphate, and folic acid based on ceRNA regulatory network as potential biomarkers for the diagnosis of pulmonary tuberculosis.

Abstract

BACKGROUND:

Pulmonary tuberculosis (TB) is a serious disease burden worldwide, and its effective early diagnosis is still facing challenges. Knowledge, acquired from multi-omics integration analysis about the association between different types of differentially expressed molecules in the plasma of TB patients and the disease traits, is anticipated to improve the accuracy of TB diagnosis through the "integrative pattern".

METHODS:

In this study, the lncRNA-miRNA-mRNA interaction network was constructed based on the competing endogenous RNA (ceRNA) hypothesis by integrating our previous data sets of lncRNA, mRNA, miRNA, and metabolites. Moreover, the key regulatory axis was established by co-expression analysis and verified at the level of metabolites.

RESULTS:

A ceRNA regulatory network consisting of 23 lncRNAs, 10 miRNAs, and 113 mRNAs was constructed. The analysis results suggested that lncRNA (OSBPL10-AS1), miRNA (has-miR-485-5p), and mRNA (SLC23A2) might be involved in the regulation of vitamin metabolism in patients with TB. Metabolite analysis showed that compared with the normal control group, TB patients had abnormal vitamin metabolism, and the expression levels of pyridoxal phosphate, pyridoxamine phosphate, and folic acid were significantly different between the two groups (p < 0.05).

CONCLUSION:

Integrated multi-omics analysis showed that vitamin metabolism disorder may be one of the pathological characteristic of TB. OSBPL10-AS1, hsa-miR-485-5p, SLC23A2, pyridoxal phosphate, pyridoxamine phosphate, and folic acid may collectively constitute the "integrative pattern" of multiple biomarkers, which may provide an accurate diagnosis of TB.

• Authors: Zhi-Bin Li, Li-Ying Shi, Yu-Shuai Han, Jing Chen, Shan-Qiang Zhang, Jia-Xi Chen, Jun Liu, Hui-Hui Tu, Qi-Qi Lu, Yi Yu, Ting-Ting Jiang, Ji-Cheng Li
• Journal: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases (Infect Genet Evol) Vol. 99 Pg. 105240 (04 2022) ISSN: 1567-7257 [Electronic] Netherlands
• PMID: 35150890 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Chemical References

• Biomarkers
• MIRN485 microRNA, human
• MicroRNAs
• RNA, Long Noncoding
• RNA, Messenger
• Vitamins
• Pyridoxal Phosphate
• Pyridoxamine
• Folic Acid
• pyridoxamine phosphate

Topics

• Biomarkers
• Folic Acid
• Gene Regulatory Networks
• Humans
• MicroRNAs (genetics)
• Pyridoxal Phosphate (genetics)
• Pyridoxamine (analogs & derivatives)
• RNA, Long Noncoding (genetics)
• RNA, Messenger (genetics)
• Tuberculosis, Pulmonary (diagnosis, genetics)
• Vitamins