Delphinidin-3-O-glucoside, an active compound of Hibiscus sabdariffa calyces, inhibits oxidative stress and inflammation in rabbits with atherosclerosis.

Abstract

CONTEXT:

Delphinidin-3-O-glucoside (DP) is a bioactive compound of Hibiscus sabdariffa L. (Malvaceae) (Roselle) calyces and exerts endothelial protection and lipid-lowering activities, which provided a basis for the prevention and treatment of cardiovascular diseases.

OBJECTIVES:

To investigate the therapeutic effects of DP against atherosclerosis.

MATERIALS AND METHODS:

A rabbit model of atherosclerosis (AS) was established by 12 weeks of a high-fat diet (HFD). The rabbits were divided into five groups: control, AS, simvastatin (4 mg/kg), and two DP groups (10 and 20 mg/kg). After treatment with DP or simvastatin by oral gavage for 12 weeks, the lipid profiles were measured. Histopathological assessment of the aorta was performed by H&E staining. Oxidative stress and inflammation-related markers were analyzed by ELISA kit and real-time RT-PCR.

RESULTS:

DP (20 mg/kg) decreased serum TG (2.36 ± 0.66 vs. 4.33 ± 0.27 mmol/L for the AS group), TC, LDL-C, and HDL-C (all p < 0.05). DP (20 mg/kg) also reduced lipid levels in the liver and aorta. DP (20 mg/kg) down-regulated the mRNA levels of IL-6, VCAM-1, and NF-κB and up-regulated the mRNA levels of GSH-PX and SOD1.

CONCLUSIONS:

This study proved that DP alleviated the HFD-induced oxidative stress and inflammation in atherosclerosis rabbits. These results provided the scientific basis for developing novel therapies.

• Authors: Bo Sun, Fangda Li, Xu Zhang, Wei Wang, Jiang Shao, Yuehong Zheng
• Journal: Pharmaceutical biology (Pharm Biol) Vol. 60 Issue 1 Pg. 247-254 (Dec 2022) ISSN: 1744-5116 [Electronic] England
• PMID: 35130117 (Publication Type: Journal Article)

Chemical References

• Anthocyanins
• Glucosides
• delphinidin 3-O-glucopyranoside

Topics

• Animals
• Anthocyanins (isolation & purification, pharmacology)
• Atherosclerosis (drug therapy, pathology)
• Diet, High-Fat
• Disease Models, Animal
• Glucosides (isolation & purification, pharmacology)
• Hibiscus (chemistry)
• Inflammation (drug therapy, pathology)
• Lipid Metabolism (drug effects)
• Male
• Oxidative Stress (drug effects)
• Rabbits