Possible Effect of Chelation Treatment on Metabolomic and Lipidomic Analysis in Lead Exposure.

Abstract

OBJECTIVE:

This study aimed to examine patients with lead poisoning in terms of metabolomic profiles and bioactive lipids (oxysterols and sphingosine 1-phosphate [S1P]) before and after chelation therapy.

METHODS:

Consent was obtained from 42 individuals diagnosed with lead poisoning and blood and urine samples were collected before and after chelation therapy. The levels of 7-ketocholesterol (7-KC), cholestan-3b,5a,6b-triol (Ctriol), and S1P were measured via LC-MS/MS. Metabolomic analysis was performed via GC-MS.

RESULTS:

7-KC and C-triol levels were detected higher before chelation therapy compared with after therapy (P < 0.001 for both). S1P levels were measured higher before the therapy. The results also showed that sphingolipid metabolism-related pathways were affected by lead toxicity as well as other related pathways.

CONCLUSION:

This preliminary study showed that lipid metabolism is affected in lead exposure and chelation therapy is effective in reversing possible damage.

• Authors: Tugce Çetin, Afshin Samadi, Tuba Reçber, Asli Karacan Dinçer, Burcu Eser, Ahmet Yalcinkaya, Emirhan Nemutlu, Yesim Öztaş, Incilay Lay, Suna Sabuncuoğlu
• Journal: Journal of occupational and environmental medicine (J Occup Environ Med) Vol. 64 Issue 5 Pg. e284-e290 (05 01 2022) ISSN: 1536-5948 [Electronic] United States
• PMID: 35121693 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2022 American College of Occupational and Environmental Medicine.

Chemical References

• Lead

Topics

• Chromatography, Liquid (methods)
• Humans
• Lead
• Lipidomics
• Metabolomics
• Tandem Mass Spectrometry (methods)