Inhibitory Effect of Oxaliplatin-loaded Engineered Milk Extracellular Vesicles on Tumor Progression.

Abstract

BACKGROUND/AIM:

Anti-cancer chemotherapy is an effective therapeutic approach. Milk extracellular vesicles (EVs) loaded with chemotherapeutics have a potential anticancer effect by acting as a drug delivery system. Thus, our study aimed to explore the effect of engineered milk extracellular vesicles.

MATERIALS AND METHODS:

To treat epidermal growth factor receptor (EGFR) expressing solid tumors, we established oxaliplatin-loaded milk EV conjugated with GE11 peptide (GE11Milk EVoxal), which has a high affinity to EGFR and assessed their anti-cancer effect in vitro and in vivo.

RESULTS:

Drug-loaded GE11Milk EVoxal showed significantly higher incorporation into EGFR expressing cancer cells compared with milk EV without GE11 conjugation (Milk EVoxal), leading to apoptosis of cancer cells. GE11Milk EVoxal also inhibited cell viability compared to milk EVoxal or oxaliplatin alone. In colorectal cancer xenograft murine model, GE11Milk EVoxal showed the maximum therapeutic effect on tumor progression. These findings indicate that GE11Milk EVoxal suppresses EGFR expressing cancer through GE11 peptide-mediated EGFR targeting and subsequently anti-cancer drug delivery.

CONCLUSION:

Anti-cancer drug-loaded engineered milk EVs might be a novel therapeutic approach for treating patients with EGFR expressing solid tumors.

• Authors: Gyeongyun Go, Hee Jung Park, Jun Hee Lee, Chul Won Yun, Sang Hun Lee
• Journal: Anticancer research (Anticancer Res) Vol. 42 Issue 2 Pg. 857-866 (Feb 2022) ISSN: 1791-7530 [Electronic] Greece
• PMID: 35093883 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Chemical References

• Antineoplastic Agents
• GE11 peptide
• Peptides
• Oxaliplatin
• EGFR protein, human
• ErbB Receptors

Topics

• Animals
• Antineoplastic Agents (administration & dosage, chemistry)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Colorectal Neoplasms (drug therapy, metabolism)
• Drug Delivery Systems
• ErbB Receptors (antagonists & inhibitors, metabolism)
• Extracellular Vesicles (chemistry)
• Humans
• Mice
• Milk (chemistry)
• Oxaliplatin (administration & dosage, chemistry)
• Peptides (administration & dosage, chemistry)
• Treatment Outcome
• Xenograft Model Antitumor Assays