Abstract | OBJECTIVES: METHODS: The epitopes of UCH-L1 protein were predicted in DNAStar software. The serum levels of different UCH-L1 epitope autoantibodies in 40 NPSLE patients, 32 SLE patients without neuropsychiatric symptoms and 21 healthy controls were determined by enzyme-linked immunosorbent assay (ELISA). Data were analysed using Pearson correlation analysis, ROC curve analysis, nonparametric Mann-Whitney test, t-test and χ2 test. RESULTS: We screened three candidate epitopes of UCH-L1 protein. The autoantibody against amino acid 58 to 69 of UCH-L1 (UCH58-69) showed highest diagnostic power in distinguishing NPSLE patients from SLE patients without neuro-psychiatric symptoms (p=0.0038). The ROC analysis showed that the specificity and sensitivity of anti-UCH58-69 were 92.3% and 37.5%, respectively. In addition, increased serum anti-UCH58-69 levels were associated with increased SLEDAI, CSF microprotein, CSF leukocyte count, ESR, AnuA, anti-dsDNA, IgG and IgM but with decrease of C3 in SLE patients. CONCLUSIONS: The serum levels of anti-UCH58-69 significantly increased in NPSLE patients compared with SLE patients without neuropsychiatric symptoms and were correlated with disease severity. Anti-UCH58-69 autoantibody may become a novel serum biomarker for NPSLE non-invasive diagnosis, which might be applicable for NPSLE early screening and diagnosis.
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Authors | Yixue Guo, Xue Li, Ruoxi Li, Yimin Li, Ziye Wang, Hongjiang Liu, Shixiong Cao, Ru Li, Yi Zhao, Qingwen Wang, Xiaolin Sun |
Journal | Clinical and experimental rheumatology
(Clin Exp Rheumatol)
Vol. 40
Issue 11
Pg. 2078-2087
(Nov 2022)
ISSN: 0392-856X [Print] Italy |
PMID | 35084329
(Publication Type: Journal Article)
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Chemical References |
- Ubiquitin Thiolesterase
- Epitopes
- Autoantibodies
- Biomarkers
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Topics |
- Humans
- Lupus Vasculitis, Central Nervous System
- Ubiquitin Thiolesterase
- Epitopes
- Autoantibodies
- Lupus Erythematosus, Systemic
- Biomarkers
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