Abstract | OBJECTIVES:
Lung cancer is the main cause of cancer death, and its incidence is increasing worldwide. The goal of this study is to evaluate in vitro and in vivo tumor targeting behavior of [99mTc]Tc -HYNIC-(Ser)3-J18 in lung carcinoma (SK-MES-1)-bearing mice. MATERIALS AND METHODS: The J18 (RSLWSDFYASASRGP) peptide was conjugated with hydrazinonicotinamide (HYNIC) via three serine amino acids as a linker at the peptide's N-terminal and then labeled with technetium-99m using tricine and tricine/ EDDA as the co- ligands. The radiolabeled peptides were assessed for in vitro receptor binding, specific binding, and saturation affinity. In vivo biodistribution studies were also performed for 99mTc-peptide 1 ( tricine co- ligand) and 99mTc-peptide 2 ( tricine/ EDDA coligands) in nude mice bearing SK-MES-1 xenograft tumors. RESULTS: In vitro studies showed high specific binding for 99mTc-peptide 1 in SKMES-1 cells compared with 99mTc-peptide 2 (11.5 vs. 4.5). The KD values for 99mTc-peptide 1 and 99mTc-peptide 2 were reported to be 3.1±0.3 nM and 3.46 ± 0.8 nM, respectively. The biodistribution study also showed high significant tumor to muscle ratios of 5.1 and 6.18 for 99mTc-peptide 1 at 1 and 2 hr after injection, respectively, while these ratios were 3.81 and 5.18 for peptide 2, respectively. CONCLUSION:
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Authors | Seyed Jalal Hosseinimehr, Soghra Farzipour, Maryam Alvandi, Zahra Shaghaghi |
Journal | Iranian journal of basic medical sciences
(Iran J Basic Med Sci)
Vol. 24
Issue 9
Pg. 1240-1246
(Sep 2021)
ISSN: 2008-3866 [Print] Iran |
PMID | 35083011
(Publication Type: Journal Article)
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