Abstract | BACKGROUND: METHODS: RESULTS:
Myelin basic protein autoantibodies were found in greater frequency in morphea (n = 50, 71.4%) compared to systemic sclerosis (n = 2, 6.7%) and healthy controls (n = 7, 20%). Patients with MBP antibodies reported pain at higher frequencies. Morphea skin biopsies, highlighted by immunohistochemistry, demonstrated increased perineural inflammation in areas of MBP expression. Immunofluorescence staining revealed an increased fluorescence signal in myelinated areas of mouse brain tissue (i.e. axons) when incubated with sera from MBP antibody-positive morphea patients compared to sera from MBP antibody-negative morphea patients. Epitope mapping revealed target epitopes for MBP autoantibodies in morphea are distinct from those reported in MS, and included fragments 11-30, 41-60, 51-70, and 91-110. CONCLUSIONS: A molecular classification of morphea based on distinct autoantibody biosignatures may be used to differentially classify morphea. We have identified anti-MBP as a potential antibody associated with morphea due to its increased expression in morphea compared to healthy controls and systemic sclerosis patients.
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Authors | Jane L Zhu, Ricardo T Paniagua, Henry W Chen, Stephanie Florez-Pollack, Elaine Kunzler, Noelle Teske, Yevgeniya Byekova Rainwater, Quan-Zhen Li, Gregory A Hosler, Wenhao Li, Denise M O Ramirez, Nancy L Monson, Heidi T Jacobe |
Journal | Journal of translational medicine
(J Transl Med)
Vol. 20
Issue 1
Pg. 41
(01 24 2022)
ISSN: 1479-5876 [Electronic] England |
PMID | 35073943
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | © 2022. The Author(s). |
Chemical References |
- Autoantibodies
- Autoantigens
- Myelin Basic Protein
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Topics |
- Animals
- Autoantibodies
- Autoantigens
- Humans
- Mice
- Multiple Sclerosis
- Myelin Basic Protein
(metabolism)
- Scleroderma, Localized
(complications)
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