Genetic Diversity and Selection of Plasmodium vivax Apical Membrane Antigen-1 in China-Myanmar Border of Yunnan Province, China, 2009-2016.

Abstract

Plasmodium vivax apical membrane antigen-1 (PvAMA-1) is an important vaccine candidate for vivax malaria. However, antigenic variation within PvAMA-1 is a major obstacle to the design of a global protective malaria vaccine. In this study, we analyzed the genetic polymorphism and selection of the PvAMA-1 gene from 152 P. vivax isolates from imported cases to China, collected in the China-Myanmar border (CMB) area in Yunnan Province (YP) during 2009-2011 (n = 71) and 2014-2016 (n = 81), in comparison with PvAMA-1 gene information from Myanmar (n = 73), collected from public data. The overall nucleotide diversity of the PvAMA-1 gene from the 152 YP isolates was 0.007 with 76 haplotypes identified (Hd = 0.958). Results from the population structure suggested three groups among the YP and Myanmar isolates with optimized clusters value of K = 7. In addition, YP (2014-2016) isolates generally lacked some K components that were commonly found in YP (2009-2011) and Myanmar. Meanwhile, PvAMA-1 domain I is found to be the dominant target of positive diversifying selection and most mutation loci were found in this domain. The mutation frequencies of D107N/A, R112K/T, K120R, E145A, E277K, and R438H in PvAMA-1 were more than 70% in the YP isolates. In conclusion, high genetic diversity and positive selection were found in the PvAMA-1 gene from YP isolates, which are significant findings for the design and development of PvAMA-1-based malaria vaccine.

• Authors: Yan-Bing Cui, Hai-Mo Shen, Shen-Bo Chen, Kokouvi Kassegne, Tian-Qi Shi, Bin Xu, Jun-Hu Chen, Jia-Hong Wu, Yue Wang
• Journal: Frontiers in cellular and infection microbiology (Front Cell Infect Microbiol) Vol. 11 Pg. 742189 ( 2021) ISSN: 2235-2988 [Electronic] Switzerland
• PMID: 35071030 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2022 Cui, Shen, Chen, Kassegne, Shi, Xu, Chen, Wu and Wang.

Chemical References

• Antigens, Protozoan
• Membrane Proteins
• Protozoan Proteins
• apical membrane antigen I, Plasmodium

Topics

• Antigens, Protozoan (genetics)
• China (epidemiology)
• Genetic Variation
• Malaria, Vivax (epidemiology, parasitology)
• Membrane Proteins (genetics)
• Myanmar (epidemiology)
• Plasmodium vivax (genetics)
• Protozoan Proteins (genetics)
• Selection, Genetic
• Sequence Analysis, DNA