Circular RNAs (
circRNAs) have been confirmed to be associated with
ischemic stroke(IS), but the involvement of exosomal
circRNAs in plasma still needs to be extensively discussed. Therefore, we aimed to investigate the expression profile of exosomal
circRNAs in plasma and the potential roles and mechanisms of exosomal
circRNAs in the pathogenesis of
ischemic stroke in the Chinese Han population. In this study, the plasma exosomal
circRNA expression profiles of three IS patients and three healthy controls were analyzed using
circRNA sequencing. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis and
circRNA-
miRNA-
mRNA regulatory network analysis were performed for the aberrantly expressed genes.
Protein-
protein interaction (PPI) networks and molecular complex detection algorithms (MCODEs) were analyzed by STRING and
Cystoscope for functional annotation and construction, respectively.
RNA-Seq analysis revealed that a total of 3540
circRNAs were aberrantly expressed in exosomes, 1177
circRNAs were significantly upregulated, and 2363
circRNAs were downregulated in IS patients compared to healthy controls. Bioinformatics analysis revealed that the parental genes of differentially expressed
circRNAs as well as the mRNAs predicted in the
circRNA-
miRNA-
mRNA regulatory network are enriched for signaling pathways associated with IS pathology, such as the MAPK signaling pathway,
lipid and
atherosclerosis,
neurotrophic factor signaling pathways, mTOR signaling pathway, the p53 signaling pathway etc. Then, 10 hub genes were identified from the PPI and module networks, including FBXW11, FBXW7, UBE2V2,
ANAPC7, CDC27, UBC, CDC5L, POLR2H, POLR2F and RBX1. Overall, the present study provides evidence of an altered plasma exosomal
circRNA expression profile and its potential function in IS. Our findings may contribute to the study of the pathogenesis of
circRNAs in IS and provide ideas for studying potential diagnostic
biomarkers and therapeutic targets for IS.