Abstract | BACKGROUND: METHODS: The compounds were analyzed by different spectroscopic techniques (FT-IR, UV-vis, 1H NMR, 13C NMR, MS) and the absorbance characteristics were determined. The cytotoxic effects of silicon phthalocyanines on MDA-MB-231 breast cancer cells and non-tumorigenic MCF-10A cells were evaluated using MTT assay. Detection of apoptotic populations was performed by Annexin V/7AAD assay. H2DCFDA dye was used to analyze intracellular reactive oxygen species. The clonogenic activity and cellular motility were analyzed by colony formation assay and in vitro scratch assay, respectively. Caspase-3, PARP1, and cleaved-PARP1 protein levels were analyzed by western blot studies. RESULTS:
Piperazine-substituted silicon phthalocyanines caused high levels of cytotoxic effects and apoptotic cell population in MDA-MB-231 cells, while low levels of cytotoxic effects were observed in MCF-10A cells. Following PDT, intense ROS formation was detected in MDA-MB-231 cells. Colony-forming capacity and cellular motility of MDA-MB-231 cells were highly restricted following PDT, whereas these effects were observed at lower levels in MCF-10A cells. Silicon phthalocyanines caused different effects on cleaved-PARP1 expressions of MDA-MB-231 and MCF-10A cells. CONCLUSION: These results suggest that piperazine-substituted silicon phthalocyanines can exert selective anti- cancer effects on breast cancer cells and activate cellular death through different molecular pathways. Hence, we believe that they may be used as effective photosensitizer agents in the future.
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Authors | Aleyna Nalçaoğlu, Ceren Sarı, İsmail Değirmencioğlu, Figen Celep Eyüpoğlu |
Journal | Photodiagnosis and photodynamic therapy
(Photodiagnosis Photodyn Ther)
Vol. 37
Pg. 102734
(Mar 2022)
ISSN: 1873-1597 [Electronic] Netherlands |
PMID | 35066132
(Publication Type: Journal Article)
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Copyright | Copyright © 2022 Elsevier B.V. All rights reserved. |
Chemical References |
- Indoles
- Organosilicon Compounds
- silicon phthalocyanine
- Piperazine
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Topics |
- Apoptosis
- Breast Neoplasms
(drug therapy)
- Cell Line, Tumor
- Female
- Humans
- Indoles
- Organosilicon Compounds
- Photochemotherapy
(methods)
- Piperazine
(pharmacology)
- Spectroscopy, Fourier Transform Infrared
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