Abstract |
The infection of coronavirus disease (COVID-19) seriously threatens human life. It is urgent to generate effective and safe specific antibodies (Abs) against the pathogenic elements of COVID-19. Mice were immunized with SARS-CoV-2 spike protein antigens: S ectodomain-1 (CoV, in short) mixed in Alum adjuvant for 2 times and boosted with CoV weekly for 6 times. A portion of mice were treated with Maotai liquor (MTL, in short) or/and heat stress (HS) together with CoV boosting. We observed that the anti-CoV Ab was successfully induced in mice that received the CoV/ Alum immunization for 2 times. However, upon boosting with CoV, the CoV Ab production diminished progressively; spleen CoV Ab-producing plasma cell counts reduced, in which substantial CoV-specific Ab-producing plasma cells (sPC) were apoptotic. Apparent oxidative stress signs were observed in sPCs; the results were reproduced by exposing sPCs to CoV in the culture. The presence of MTL or/and HS prevented the CoV-induced oxidative stress in sPCs and promoted and stabilized the CoV Ab production in mice in re-exposure to CoV. In summary, CoV/ Alum immunization can successfully induce CoV Ab production in mice that declines upon reexposure to CoV. Concurrent administration of MTL/HS stabilizes and promotes the CoV Ab production in mice.
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Authors | Li-Hua Mo, Xiang-Qian Luo, Ke Ma, Jian-Bo Shao, Guo-Hao Zhang, Zhi-Gang Liu, Da-Bo Liu, Huan-Ping Zhang, Ping-Chang Yang |
Journal | Oxidative medicine and cellular longevity
(Oxid Med Cell Longev)
Vol. 2022
Pg. 5397733
( 2022)
ISSN: 1942-0994 [Electronic] United States |
PMID | 35047106
(Publication Type: Journal Article)
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Copyright | Copyright © 2022 Li-Hua Mo et al. |
Chemical References |
- Adjuvants, Immunologic
- Alum Compounds
- Antibodies, Neutralizing
- Antibodies, Viral
- COVID-19 Vaccines
- Reactive Oxygen Species
- STAT1 Transcription Factor
- Spike Glycoprotein, Coronavirus
- Stat1 protein, mouse
- spike protein, SARS-CoV-2
- aluminum sulfate
- Sod1 protein, mouse
- Superoxide Dismutase-1
- Jak2 protein, mouse
- Janus Kinase 2
- Ace2 protein, mouse
- Angiotensin-Converting Enzyme 2
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Topics |
- Adjuvants, Immunologic
- Alcoholic Beverages
- Alum Compounds
- Angiotensin-Converting Enzyme 2
(physiology)
- Animals
- Antibodies, Neutralizing
(biosynthesis, blood)
- Antibodies, Viral
(biosynthesis, blood)
- Apoptosis
- COVID-19
(enzymology, immunology)
- COVID-19 Vaccines
(immunology)
- Heat-Shock Response
- Immunization, Secondary
- Immunogenicity, Vaccine
- Janus Kinase 2
(physiology)
- Male
- Mice
- Mice, Inbred C57BL
- Oxidative Stress
- Plasma Cells
(drug effects, immunology, pathology)
- Reactive Oxygen Species
(metabolism)
- SARS-CoV-2
(physiology)
- STAT1 Transcription Factor
(physiology)
- Signal Transduction
- Specific Pathogen-Free Organisms
- Spike Glycoprotein, Coronavirus
(immunology)
- Superoxide Dismutase-1
(physiology)
- Vaccination
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