The interplay between estrogen receptor beta and protein kinase C, a crucial collaboration for medulloblastoma cell proliferation and invasion.

Abstract

Medulloblastoma (MB) is the most common and aggressive pediatric intracranial tumor. Estrogen receptor β (ERβ) expression correlates with MB development and its phosphorylation modifies its transcriptional activity in a ligand-dependent or independent manner. Using in silico tools, we have identified several residues in ERβ protein as potential targets of protein kinases C (PKCs) α and δ. Using Daoy cells, we observed that PKCα and PKCδ associate with ERβ and induce its phosphorylation. The activation of ERβ promotes MB cells proliferation and invasion, and PKCs downregulation dysregulates these steroid receptor mediated processes. Our data suggest that these kinases may play a crucial role in the regulation of the ERβ transcriptional activity. Overexpression of both PKCα and PKCδ in MB biopsies samples supports their relevance in MB progression.

• Authors: Rubí Hernández-Rojas, Carolina Jiménez-Arellano, Marisol de la Fuente-Granada, David Ordaz-Rosado, Rocío García-Becerra, Pedro Valencia-Mayoral, Lourdes Álvarez-Arellano, Pilar Eguía-Aguilar, Marco A Velasco-Velázquez, Aliesha González-Arenas
• Journal: Cellular signalling (Cell Signal) Vol. 92 Pg. 110246 (04 2022) ISSN: 1873-3913 [Electronic] England
• PMID: 35033667 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2022 Elsevier Inc. All rights reserved.

Chemical References

• Estrogen Receptor alpha
• Estrogen Receptor beta
• PRKCA protein, human
• Protein Kinase C
• Protein Kinase C-alpha
• Protein Kinase C-delta

Topics

• Cell Line, Tumor
• Cell Proliferation
• Cerebellar Neoplasms
• Child
• Estrogen Receptor alpha
• Estrogen Receptor beta (genetics, metabolism)
• Humans
• Medulloblastoma (genetics, metabolism)
• Protein Kinase C
• Protein Kinase C-alpha (metabolism)
• Protein Kinase C-delta (metabolism)