Abstract | BACKGROUND: METHODS: C4-2B and PC3 prostate cancer cells were cultured in media supplemented with fetal calf serum (FCS), charcoal-stripped FCS (CS-FCS), lipoprotein-deficient FCS (LPDS), or charcoal-stripped LPDS (CS-LPDS) and analyzed by a variety of biochemical techniques. Cell viability and proliferation were measured by MTT assay and Incucyte, respectively. RESULTS: Reducing lipoprotein availability led to a reduction in cholesteryl ester levels and cell growth in C4-2B and PC3 cells, with concomitant reductions in PI3K/mTOR and p38MAPK signaling. This reduced growth in LPDS-containing media was fully recovered by supplementation of exogenous low-density lipoprotein ( LDL), but LDL only partially rescued growth of cells cultured with CS-LPDS. This growth pattern was not associated with changes in androgen receptor signaling but rather increased p38MAPK and MEK1/ERK/MSK1 activation. The ability of LDL supplementation to rescue cell growth required cholesterol esterification as well as cholesteryl ester hydrolysis activity. Further, growth of cells cultured in low androgen levels (CS-FCS) was suppressed when cholesteryl ester hydrolysis was inhibited. CONCLUSIONS:
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Authors | Nikki L Raftopulos, Tinashe C Washaya, Andreas Niederprüm, Antonia Egert, Mariam F Hakeem-Sanni, Bianca Varney, Atqiya Aishah, Mariya L Georgieva, Ellinor Olsson, Diandra Z Dos Santos, Zeyad D Nassar, Blake J Cochran, Shilpa R Nagarajan, Meghna S Kakani, Jordan F Hastings, David R Croucher, Kerry-Anne Rye, Lisa M Butler, Thomas Grewal, Andrew J Hoy |
Journal | Cancer & metabolism
(Cancer Metab)
Vol. 10
Issue 1
Pg. 1
(Jan 15 2022)
ISSN: 2049-3002 [Print] England |
PMID | 35033184
(Publication Type: Journal Article)
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Copyright | © 2022. The Author(s). |
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