Transcription factor NFIC functions as a tumor suppressor in lung squamous cell carcinoma progression by modulating lncRNA CASC2.

Abstract

Nuclear factor I (NFI) family is emerging found playing oncogenic or tumor-suppressive potential in cancers. However, the function and underlying mechanisms of NFIC, in the progression of Lung Squamous Cell Carcinoma (LUSC) remain unclear. Therefore, this study aims to probe into the function of NFIC in the development of LUSC. In the present study, we reported that NFIC was low expressed in human LUSC tissues and cell lines. NFIC inhibited LUSC cell proliferation and promoted cell apoptosis in vitro and in vivo. Moreover, NFIC also inhibited LUSC cell migration and invasion. Furthermore, we found that there were binding sites between lncRNA cancer susceptibility candidate 2 (CASC2) and NFIC, whose relationship was confirmed by the luciferase reporter assay. The expression of CASC2 and the expression of NFIC were positively correlated, and the function of CASC2 overexpression is similar to that of NFIC overexpression, which suggested that CASC2 may play a key role in LUSC development. Our study provided a new perspective for NFIC acting as an antioncogene in LUSC tumorigenesis, and NFIC and CASC2 may serve as novel potential targets for the treatment of LUSC.

• Authors: Hong Zhang, Zhilin Luo, JianMing Tang, Jie Tian, Yajie Xiao, Chao Sun, Tianhu Wang
• Journal: Cell cycle (Georgetown, Tex.) (Cell Cycle) Vol. 21 Issue 1 Pg. 63-73 (01 2022) ISSN: 1551-4005 [Electronic] United States
• PMID: 34985387 (Publication Type: Journal Article)

Chemical References

• MicroRNAs
• NFI Transcription Factors
• NFIC protein, human
• RNA, Long Noncoding
• Tumor Suppressor Proteins
• long non-coding RNA CASC2, human

Topics

• Carcinoma, Non-Small-Cell Lung (genetics)
• Carcinoma, Squamous Cell (genetics)
• Cell Line, Tumor
• Cell Proliferation (genetics)
• Gene Expression Regulation, Neoplastic
• Genes, Tumor Suppressor
• Humans
• Lung (metabolism)
• Lung Neoplasms (genetics, pathology)
• MicroRNAs (genetics)
• NFI Transcription Factors (genetics)
• RNA, Long Noncoding (genetics, metabolism)
• Tumor Suppressor Proteins (genetics, metabolism)