Long noncoding RNA has been reported to play important role in various disease. However, the function of
lncRNA in age-related
hearing loss still unclear. The aim of our study is to investigate the function and mechanism of
lncRNA Gm44593 in AHL.
ATP content,
JC-1 assay, mitochondrial content, cell death rates and dual-
luciferase reporter assay were performed to assess the function of
lncRNA Gm44593 in HEI-OC1 cells. The expression of
lncRNA Gm44593 was significantly upregulated upon H2O2 and
starvation treatment. Overexpression of
lncRNA Gm44593 manifestly reduced the cell death rates. The
ATP content,
mtDNA content and mitochondrial membrane potential were alleviated upon overexpression of
lncRNA Gm44593. We also proved that
miR-29b is the direct target of
lncRNA Gm44593. Overexpression of
miR-29b completely restored the effect induced by
lncRNA Gm44593. In addition, we provided evidences that WNK1 is the direct target of
miR-29b. Our research uncovers a potential role of
lncRNA Gm44593 in age-related
hearing loss. We provide new insights into potential therapeutic targets for the amelioration of age-related
hearing loss.