The objective of this study was to evaluate effects of
sodium-butyrate supplementation on gastrointestinal function and the inflammatory response to ruminal
acidosis (RA) challenge in cows. Four nonlactating cows with a rumen
cannula were assigned to two treatments in a crossover design. Treatments were ruminal administration of
sodium-butyrate (BUT) or control (CON).
Sodium-butyrate was provided as Gustor BP70 and administered at a
butyrate dose of 0.04% per kg
body weight. The CON premix was made by replacing
sodium-butyrate with
wheat bran. Experimental periods were 28 days long with 21-day washout period separating the treatments. On Day 25 of each period, corn
starch was ruminally administered at 0.7% per kg
body weight as RA challenge. After RA challenge, ruminal pH was lower, and
endotoxin concentration was higher for cows provided with BUT than those with CON, but the increase in fecal
starch and the decrease in fecal pH were attenuated by BUT. The effect of
butyrate supplementation on serum
lipopolysaccharide-binding protein after RA challenge was not found. From these findings,
butyrate supplementation mitigated rectal
acidosis by reducing the flux of fermentable
carbohydrate into the large intestine. An anti-inflammatory effect of
butyrate was not observed, possibly due to lower pH and higher
endotoxin concentration in the rumen.