[Quantification of SS-B autoantibodies in patient sera using highly purified human SS-B antigens and their clinical interpretation].
|
| Abstract |
For the identification of SS-B autoantibodies in the sera of patients with rheumatic diseases (n = 319) a sensitive and specific enzyme-linked immunosorbent assay was developed using highly purified human SS-B antigen as a reference antigen. In 30/319 patients' sera SS-B autoantibodies were detected. In this group, the ratio female : male patients was outstandingly high (29:1), the serum of the single male patient exhibiting the lowest titer of SS-B autoantibodies. The highest portion of anti-SS-B positive sera was observed in the group of patients with systemic Lupus erythematosus (48%) followed by the groups with undifferentiated connective tissue disease (17%), Sjögren-syndrome (13%) and rheumatoid arthritis (6%). The protein components reacting with human SS-B autoantibodies were characterized in permanent cell lines of various species. In each of 9 human cell lines only one component with a molecular weight of 49 kD reacted; in cell lines of other species analogously, only one component reacted, showing slightly different molecular weights depending on the species. The SS-B antigen isolated from human cells contained at least 7 isoelectric variants, all reacting with human SS-B autoantibodies.
|
|---|---|
| Authors | J Pfeifle, J M Wolff, F A Anderer, M Franke |
| Journal | Zeitschrift fur Rheumatologie (Z Rheumatol) 1986 Nov-Dec Vol. 45 Issue 6 Pg. 296-303 ISSN: 0340-1855 [Print] Germany |
| Vernacular Title | Quantifizierung von SS-B Autoantikörpern in Patientenseren mittels hochgereinigtem humanem SS-B Antigen und ihre klinische Interpretation. |
| PMID | 3493602 (Publication Type: English Abstract, Journal Article) |
| Chemical References |
|
| Topics |
|
Join CureHunter, for free Research Interface BASIC access!
Realize the full power of the drug-disease research graph!