Mechanisms of Progression and Heterogeneity in Multiple Nodules of Lung Adenocarcinoma.

Abstract

Lung cancer remains the leading cause of cancer-related death worldwide. Lung adenocarcinoma (LUAD) is thought to be caused by precursor lesions of atypical adenoma-like hyperplasia and may have extensive in situ growth before infiltration. To explore the relevant factors in heterogeneity and evolution of lung adenocarcinoma subtypes, the authors perform single-cell RNA sequencing (scRNA-seq) on tumor and normal tissue from five multiple nodules' LUAD patients and conduct a thorough gene expression profiling of cancer cells and cells in their microenvironment at single-cell level. This study gives a deep understanding of heterogeneity and evolution in early glandular neoplasia of the lung. This dataset leads to discovery of the changes in the immune microenvironment during the development of LUAD, and the development process from adenocarcinoma in situ (AIS) to invasive adenocarcinoma (IAC). This work sheds light on the direction of early tumor development and whether they are homologous.

• Authors: Yayi He, Xiaogang Liu, Hao Wang, Liang Wu, Minlin Jiang, Haoyue Guo, Junjie Zhu, Shengyu Wu, Hui Sun, Shanhao Chen, Yuming Zhu, Caicun Zhou, Yang Yang
• Journal: Small methods (Small Methods) Vol. 5 Issue 6 Pg. e2100082 (06 2021) ISSN: 2366-9608 [Electronic] Germany
• PMID: 34927899 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2021 Wiley-VCH GmbH.

Topics

• Adenocarcinoma (genetics)
• Adenocarcinoma in Situ (genetics)
• Adenocarcinoma of Lung (genetics)
• Gene Expression Profiling
• Humans
• Lung Neoplasms (genetics)
• Tumor Microenvironment (genetics)