Abstract | AIMS: MAIN METHODS: KEY FINDINGS: Both ADSCs and ADSC-CM could improve the damage in the ultrastructure of hepatocytes. ADSC-CM significantly decreased the protein expression of GRP78, ATF6, XBP1, p-eIF2α, ATF4, p-JNK and CHOP. Oil red O staining revealed that the degree of hepatocyte steatosis was also significantly reduced after treatment with ADSC-CM. In addition, ADSC-CM remarkably decreased TG, TC, HDL and ACC1 level in liver tissues, while enhanced VLDL content. Finally, SREBP1, SCAP, FASN, ACC1, HMGCR and HMGCS1 mRNA expression was also markedly downregulated in liver tissues. SIGNIFICANCE: Injection of ADSC-CM into the hepatic parenchymal could represent a novel cell-free therapeutic approach to improve HIRI combined with hepatectomy injury. The inhibition of ERS and the improvement of lipid metabolism in the hepatocytes might be a potential mechanism used by ADSC-CM to prevent liver injury from HIRI combined with hepatectomy.
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Authors | Qianzhen Zhang, Xiaoning Liu, Chenxi Piao, Zhihui Jiao, Yajun Ma, Yue Wang, Tao Liu, Jiayuan Xu, Hongbin Wang |
Journal | Life sciences
(Life Sci)
Vol. 289
Pg. 120212
(Jan 15 2022)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 34896163
(Publication Type: Journal Article)
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Copyright | Copyright © 2021 Elsevier Inc. All rights reserved. |
Chemical References |
- Culture Media, Conditioned
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Topics |
- Adipose Tissue
(metabolism)
- Animals
- Culture Media, Conditioned
(pharmacology)
- Endoplasmic Reticulum Stress
- Hepatectomy
- Lipid Metabolism
- Liver
(metabolism)
- Liver Failure
(drug therapy, metabolism)
- Liver Transplantation
- Mesenchymal Stem Cells
(metabolism)
- Reperfusion Injury
(drug therapy, metabolism)
- Swine
- Swine, Miniature
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