The use of immunoblotting and immunoprecipitation of (U) small nuclear ribonucleoproteins in the analysis of sera of patients with mixed connective tissue disease and systemic lupus erythematosus. A cross-sectional, longitudinal study.

Abstract

Antibodies to ribonucleoproteins (RNP) and to the Sm antigen in sera from patients with mixed connective tissue disease (MCTD) and systemic lupus erythematosus were studied using the techniques of immunoblotting and immunoprecipitation of U small nuclear RNPs. A cross-sectional study indicated that antibodies reacting with a 68K protein were associated with anti-RNP specificity in MCTD, but rarely occurred in systemic lupus erythematosus patients' sera. A longitudinal study demonstrated the persistence of MCTD blotting patterns over many years, and the subsequent disappearance of those specificities in sera from patients who were in prolonged remission.

• Authors: I Pettersson, G Wang, E I Smith, H Wigzell, E Hedfors, J Horn, G C Sharp
• Journal: Arthritis and rheumatism (Arthritis Rheum) Vol. 29 Issue 8 Pg. 986-96 (Aug 1986) ISSN: 0004-3591 [Print] United States
• PMID: 3488746 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies
• Antibodies, Antinuclear
• Ribonucleoproteins

Topics

• Antibodies (immunology)
• Antibodies, Antinuclear (immunology)
• Electrophoresis, Polyacrylamide Gel
• Humans
• Longitudinal Studies
• Lupus Erythematosus, Systemic (blood)
• Mixed Connective Tissue Disease (blood)
• Ribonucleoproteins (blood, immunology)