Identification of ACOT13 and PTGER2 as novel candidate genes of autosomal dominant polycystic kidney disease through whole exome sequencing.

Abstract

BACKGROUND:

Autosomal dominant polycystic kidney disease (ADPKD) is the most common monogenic kidney disorder. Half of the patients would slowly progress to end-stage renal disease. However, the potential target for ADPKD treatment is still lacking.

METHODS:

Four ADPKD patients and two healthy family members were included in this study. The peripheral blood samples were obtained and tested by the whole exome sequencing (WES). The autosomal mutations in ADPKD patients were retained as candidate sites. The Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, and protein-protein interaction network (PPI) analyses were performed by clusterProfiler R package. A dataset containing 18 ADPKD patients and three normal samples were downloaded from the Gene Expression Omnibus (GEO) database and analyzed using the limma R package.

RESULTS:

A total of six mutant genes were identified based on the dominant genetic pattern and most of them had not been reported to be associated with ADPKD. Furthermore, 19 harmful genes were selected according to the harmfulness of mutation. GO and KEGG enrichment analyses showed that the processes of single-organism cellular process, response to stimulus, plasma membrane, cell periphery, and anion binding as well as cyclic adenosine monophosphate (cAMP) signaling pathway and pathways in cancer were significantly enriched. Through integrating PPI and gene expression analyses, acyl-CoA thioesterase 13 (ACOT13), which has not been reported to be related to ADPKD, and prostaglandin E receptor 2 (PTGER2) were identified as potential genes associated with ADPKD.

CONCLUSIONS:

Through combination of WES, gene expression, and PPI network analyses, we identified ACOT13 and PTGER2 as potential ADPKD-related genes.

• Authors: Na Du, Dan Dong, Luyao Sun, Lihe Che, Xiaohua Li, Yong Liu, Bin Wang
• Journal: European journal of medical research (Eur J Med Res) Vol. 26 Issue 1 Pg. 142 (Dec 09 2021) ISSN: 2047-783X [Electronic] England
• PMID: 34886911 (Publication Type: Journal Article)
• Copyright: © 2021. The Author(s).

Chemical References

• PTGER2 protein, human
• Receptors, Prostaglandin E, EP2 Subtype
• RNA
• ACOT13 protein, human
• Thiolester Hydrolases

Topics

• Female
• Gene Expression Regulation
• Humans
• Male
• Pedigree
• Polycystic Kidney, Autosomal Dominant (diagnosis, genetics, metabolism)
• RNA (genetics)
• Receptors, Prostaglandin E, EP2 Subtype (biosynthesis, genetics)
• Thiolester Hydrolases (biosynthesis, genetics)
• Tomography, X-Ray Computed
• Exome Sequencing (methods)