Anti-SARS-CoV-2 antibodies elicited by COVID-19 mRNA vaccine exhibit a unique glycosylation pattern.

Abstract

Messenger RNA-based vaccines against COVID-19 induce a robust anti-SARS-CoV-2 antibody response with potent viral neutralization activity. Antibody effector functions are determined by their constant region subclasses and by their glycosylation patterns, but their role in vaccine efficacy is unclear. Moreover, whether vaccination induces antibodies similar to those in patients with COVID-19 remains unknown. We analyze BNT162b2 vaccine-induced IgG subclass distribution and Fc glycosylation patterns and their potential to drive effector function via Fcγ receptors and complement pathways. We identify unique and dynamic pro-inflammatory Fc compositions that are distinct from those in patients with COVID-19 and convalescents. Vaccine-induced anti-Spike IgG is characterized by distinct Fab- and Fc-mediated functions between different age groups and in comparison to antibodies generated during natural viral infection. These data highlight the heterogeneity of Fc responses to SARS-CoV-2 infection and vaccination and suggest that they support long-lasting protection differently.

• Authors: Inbal Farkash, Tali Feferman, Noy Cohen-Saban, Yahel Avraham, David Morgenstern, Grace Mayuni, Natasha Barth, Yaniv Lustig, Liron Miller, Dror S Shouval, Asaf Biber, Ilya Kirgner, Yishai Levin, Rony Dahan
• Journal: Cell reports (Cell Rep) Vol. 37 Issue 11 Pg. 110114 (12 14 2021) ISSN: 2211-1247 [Electronic] United States
• PMID: 34883043 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

Chemical References

• Antibodies, Viral
• COVID-19 Vaccines
• Immunoglobulin G
• Spike Glycoprotein, Coronavirus
• Vaccines, Synthetic
• mRNA Vaccines
• BNT162 Vaccine

Topics

• Adult
• Aged
• Antibodies, Viral (immunology)
• BNT162 Vaccine (immunology)
• COVID-19 (immunology)
• COVID-19 Vaccines (metabolism)
• Female
• Glycosylation (drug effects)
• Humans
• Immunoglobulin G (immunology, metabolism)
• Israel (epidemiology)
• Male
• Middle Aged
• SARS-CoV-2 (immunology)
• Spike Glycoprotein, Coronavirus (immunology)
• Vaccination (methods)
• Vaccine Efficacy
• Vaccines, Synthetic (immunology, metabolism)
• mRNA Vaccines (immunology, metabolism)