p-phenylenediamine (
PPD) is a common component of
hair dye known to induce immediate
allergy, even acute
dermatitis and
contact dermatitis. MAS-related
G protein coupled receptor-X2 (MRGPRX2) in mast cells (MCs) mediates small molecular substances-induced pseudo-
allergic reactions. However, the role of MRGPRX2 in
PPD-induced immediate contact
allergy needs further exploration. The aim of this study was to investigate whether
PPD activates MCs via MRGPRX2 and induces immediate
allergies that contribute to
contact dermatitis. Wild-type (WT) and kitw-sh/w-sh mice (MUT) were treated with
PPD to observe local
inflammation and MC degranulation in vivo. The release of inflammatory mediators was measured in vitro.
Histamine 1 receptor (H1R)-/- mice were used to analyze itch type.
PPD caused immediate contact
allergy in WT mice, induced scratching, and local inflammatory reactions, while exhibiting minimal effects on MUT mice.
PPD did not induce histamine release, but induced significant
tryptase release in vivo and in vitro.
PPD activated MRGPRX2 to induce MC degranulation in vitro.
PPD caused immediate contact
allergy in WT mice, induced scratching and local inflammatory reactions, while exhibited minimal effect on MUT mice.
PPD did not induce histamine release, while induced significant
tryptase release in vivo and in vitro.
PPD induced immediate contact
allergy by MCs activation via MRGPRX2 and lead to
tryptase release. The scratching times showed no significant difference in WT mice or H1R-/- mice, which indicated
PPD caused non-histaminergic itch. The results showed that
PPD activated MCs via MRGPRX2 and induced immediate contact
allergy, leading to the release of
tryptase without monoamine release, which might induce non-histaminergic itch.