Abstract | OBJECTIVE: MATERIALS AND METHODS: 30 paired laryngeal tissue specimens (neoplastic and non-neoplastic) were included in the study. Nucleic acid isolation and cDNA synthesis was performed according to the manufacturer's protocol. EBV DNA and expression of lytic (BZLF1) and latent (LMP1) forms of infection were assessed in PCR assays; expression levels of H19 and EZH2 were quantified in qRT-PCR. Data was analysed using GraphPad Prism 5.0. RESULTS: Higher H19 relative expression in neoplastic vs paired non-neoplastic samples was found (p < 0.0001) with a significant increase in EBV DNA positive neoplasms (p = 0.0434). An inverse correlation between H19 and EZH2 expression levels was noticed in EBV positive cases. Additionally, increased levels of H19 in LMP1 positive samples compared with those positive for BZLF1 was found (p = 0.0593). CONCLUSIONS:
|
Authors | Irina E Hotoboc, Alina Fudulu, Raluca Grigore, Serban Bertesteanu, Irina Huica, Iulia V Iancu, Anca Botezatu, Coralia Bleotu, Gabriela Anton |
Journal | Acta otorhinolaryngologica Italica : organo ufficiale della Societa italiana di otorinolaringologia e chirurgia cervico-facciale
(Acta Otorhinolaryngol Ital)
Vol. 41
Issue 6
Pg. 537-543
(Dec 2021)
ISSN: 1827-675X [Electronic] Italy |
PMID | 34825669
(Publication Type: Journal Article)
|
Copyright | Copyright © 2021 Società Italiana di Otorinolaringoiatria e Chirurgia Cervico-Facciale, Rome, Italy. |
Chemical References |
- H19 long non-coding RNA
- RNA, Long Noncoding
- EZH2 protein, human
- Enhancer of Zeste Homolog 2 Protein
|
Topics |
- Carcinoma
(genetics)
- Enhancer of Zeste Homolog 2 Protein
(genetics)
- Epstein-Barr Virus Infections
(complications, genetics)
- Herpesvirus 4, Human
(genetics)
- Humans
- Laryngeal Neoplasms
(genetics)
- RNA, Long Noncoding
(genetics)
|