Simultaneous analysis of serum α2,3-linked sialylation and core-type fucosylation of prostate-specific antigen for the detection of high-grade prostate cancer.

Abstract

BACKGROUND:

Altered prostate-specific antigen (PSA) glycosylation patterns can be useful biomarkers in detecting high-grade prostate cancer (HGPC). The microfluidic immunoassay system can analyse α2,3-linked sialylated PSA (α2,3-Sia-PSA) and α1,6-linked fucosylated PSA (α1,6-Fuc-PSA) using different lectins, Mackkia amurensis agglutinin and Pholiota squarrosa lectin, respectively. Here, we investigated the diagnostic value of simultaneous analysis of α2,3-Sia-PSA and α1,6-Fuc-PSA for the detection of HGPC.

METHODS:

Men with serum PSA levels of 4-20 ng/mL who underwent prostate biopsy were included. The model to predict HGPC (Gleason grade ≥2) was constructed by multivariate logistic regression analysis, in combination with α2,3-Sia-PSA and α1,6-Fuc-PSA (SF index).

RESULTS:

In the development cohort (n = 150), the SF index showed good discrimination for HGPC (area under the receiver-operating curve (AUC) 0.842; 95% confidence interval (CI) 0.782-0.903), compared to the single PSA test (AUC 0.632, 95% CI 0.543-0.721), α2,3-Sia-PSA (AUC 0.711, 95% CI 0.629-0.793) and α1,6-Fuc-PSA (AUC 0.738, 95% CI 0.657-0.819). Decision-curve analysis showed the superior benefit of the SF index. In the validation cohort (n = 57), the SF index showed good discrimination for HGPC (AUC 0.769, 95% CI 0.643-0.895).

CONCLUSIONS:

The SF index could differentiate HGPC, providing useful information for decision making for prostate biopsy in men with abnormal PSA levels.

• Authors: Koji Hatano, Tohru Yoneyama, Shingo Hatakeyama, Eisuke Tomiyama, Mutsumi Tsuchiya, Mitsuhisa Nishimoto, Kazuhiro Yoshimura, Eiji Miyoshi, Hirotsugu Uemura, Chikara Ohyama, Norio Nonomura, Kazutoshi Fujita
• Journal: British journal of cancer (Br J Cancer) Vol. 126 Issue 5 Pg. 764-770 (03 2022) ISSN: 1532-1827 [Electronic] England
• PMID: 34802050 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2021. The Author(s), under exclusive licence to Springer Nature Limited.

Chemical References

• Biomarkers, Tumor
• Prostate-Specific Antigen

Topics

• Adult
• Aged
• Aged, 80 and over
• Biomarkers, Tumor (blood, chemistry)
• Glycosylation
• Humans
• Logistic Models
• Male
• Microfluidic Analytical Techniques (instrumentation)
• Middle Aged
• Neoplasm Grading
• Prostate-Specific Antigen (blood, chemistry)
• Prostatic Neoplasms (blood, diagnosis)
• Sensitivity and Specificity