LncRNA TMPO-AS1 serves as a sponge for miR-4731-5p modulating breast cancer progression through FOXM1.

Abstract

OBJECTIVE:

To investigate the function of IncRNA-TMPO-AS1 in breast cancer (BC) and to further explore its molecular mechanism.

METHODS:

TMPO-AS1, miR-4731-5p and FOXM1 were quantitatively determined using qRT-PCR. CCK-8 assays, plate cloning experiments, wound healing and Transwell assays, and flow cytometry were used to assess the biological behaviors of BC cells. Dual-luciferase reporter assays were used to assess the interactions between TMPO-AS1 and its downstream targets. The apoptosis and cell cycle-related proteins were quantitatively determined using Western blot.

RESULTS:

In the BC tissues and cells, TMPO-AS1 was significantly increased (P<0.05). Functional studies suggest that the knockdown of TMPO-AS1 tremendously restrains tumor cell growth and migration (P<0.05). Mechanically, TMPO-AS1 negatively regulates miR-4731-5p and influences the progression of BC through the miR-4731-5p/FOXM1 axis.

CONCLUSION:

LncRNA TMPO-AS1spongess miR-4731-5p to modulate BC progression through FOXM1.

• Authors: Ying Wang, Jun Ma, Ruiqing Li, Xiaokang Gao, Hongli Wang, Guoqin Jiang
• Journal: American journal of translational research (Am J Transl Res) Vol. 13 Issue 10 Pg. 11094-11106 ( 2021) ISSN: 1943-8141 [Print] United States
• PMID: 34786045 (Publication Type: Journal Article)
• Copyright: AJTR Copyright © 2021.