The protective effect of manganese superoxide dismutase from thermophilic bacterium HB27 on hydrochloric acid-induced chemical cystitis in rats.

Abstract

PURPOSE:

To evaluate the effects of manganese superoxide dismutase (Mn-SOD) from thermophilic bacterium HB27 (name as Tt-SOD) on chemical cystitis.

METHODS:

Control and experimental rats were infused by intravesical saline or hydrochloric acid (HCl) on the first day of the experiments. Saline, sodium hyaluronate (SH) or Tt-SOD were infused intravesically once a day for three consequent days. On the fifth day, the rats were weighted and sacrificed following a pain threshold test. The bladder was harvested for histological and biochemical analyses.

RESULTS:

Tt-SOD could reduce the bladder index, infiltration of inflammatory cells in tissues, serum inflammatory factors and SOD levels, mRNA expression of inflammatory factors in tissues, and increase perineal mechanical pain threshold and serum MDA and ROS levels in HCl-induced chemical cystitis. Furthermore, Tt-SOD alleviated inflammation and oxidative stress by the negative regulation of the NF-κB p65 and p38 MAPK signaling pathway.

CONCLUSIONS:

Intravesical instillation of Tt-SOD provides protective effects against HCl-induced cystitis.

• Authors: Nai-Wen Chen, Jin-Lai Gao, Hai-Long Li, Hong Xu, Ling-Feng Wu, Fan-Guo Meng, Wei Chen, Yi-Fang Cao, Wen-Hua Xie, Xiao-Qin Zhang, Shi-Hui Liu, Jing Jin, Yi He, Jian-Wei Lv
• Journal: International urology and nephrology (Int Urol Nephrol) Vol. 54 Issue 7 Pg. 1681-1691 (Jul 2022) ISSN: 1573-2584 [Electronic] Netherlands
• PMID: 34783980 (Publication Type: Journal Article)
• Copyright: © 2021. The Author(s).

Chemical References

• Bacterial Proteins
• Superoxide Dismutase
• Hydrochloric Acid

Topics

• Animals
• Bacterial Proteins (therapeutic use)
• Cystitis (chemically induced, therapy)
• Hydrochloric Acid (adverse effects)
• Inflammation (metabolism)
• Rats
• Rats, Sprague-Dawley
• Superoxide Dismutase (therapeutic use)
• Urinary Bladder (pathology)