Abstract |
Oxalate-induced oxidative stress causes damage to cells, accompanied with renal deposition of calcium oxalate crystals. Recent studies have highlighted the extensive functions of microRNAs ( miRNAs) in various processes, including cellular responses to oxidative stress. Hence, this study was intended to analyze the role of miR-204 in the calcium oxalate kidney-stone formation and the underlying mechanism. In silico analysis was performed to determine the miRNA/ mRNA interaction involved in calculus, while dual- luciferase reporter assay was conducted for validation. A calcium oxalate kidney-stone model was established by H2O2 induction in RTEC HK-2 cells, in which the expression of miR-204 was examined. Gain- and loss-of-function approaches were employed to alter the expression of miR-204/MUC4 so as to assess the detailed role of miR-204 in oxidative stress injury in renal tubular epithelial cells (RTECs) and calcium oxalate kidney-stone formation. MUC4, an up-regulated gene in H2O2-induced HK-2 cells, was a target of MUC4. miR-204 functionally targeted MUC4 and blocked the ERK pathway activation. Furthermore, up-regulated miR-204 contributed to promotion of RTEC proliferation and suppression of ROS levels, RTEC apoptosis as well as formation of calcium oxalate crystal. Taken together, miR-204 impairs MUC4-dependent activation of the ERK signaling pathway and consequently ameliorates oxidative stress damage to RTECs and prevents calcium oxalate kidney-stone formation.
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Authors | Zhijuan Xie, Jianying Chen, Zhong Chen |
Journal | Urolithiasis
(Urolithiasis)
Vol. 50
Issue 1
Pg. 1-10
(Feb 2022)
ISSN: 2194-7236 [Electronic] Germany |
PMID | 34783868
(Publication Type: Journal Article)
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Copyright | © 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. |
Chemical References |
- MicroRNAs
- Calcium Oxalate
- Hydrogen Peroxide
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Topics |
- Calcium Oxalate
(metabolism)
- Epithelial Cells
(metabolism)
- Humans
- Hydrogen Peroxide
(metabolism, toxicity)
- Kidney
(metabolism)
- MAP Kinase Signaling System
- MicroRNAs
(genetics, metabolism)
- Oxidative Stress
- Signal Transduction
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