Ketoprofen Combined with UVA Irradiation Exerts Higher Selectivity in the Mode of Action against Melanotic Melanoma Cells than against Normal Human Melanocytes.

Abstract	Malignant melanoma is responsible for the majority of skin cancer-related deaths. The methods of cancer treatment include surgical removal, chemotherapy, immunotherapy, and targeted therapy. However, neither of these methods gives satisfactory results. Therefore, the development of new anticancer therapeutic strategies is very important and may extend the life span of people suffering from melanoma. The aim of this study was to examine the effect of ketoprofen (KTP) and UVA radiation (UVAR) therapy on cell proliferation, apoptosis, and cell cycle distribution in both melanotic melanoma cells (COLO829) and human melanocytes (HEMn-DP) in relation to its supportive effect in the treatment of melanoma. The therapy combining the use of pre-incubation with KTP and UVAR causes a significant increase in the anti-proliferative properties of ketoprofen towards melanoma cells and the co-exposure of melanotic melanoma cells induced apoptosis shown as the mitochondrial membrane breakdown, cell-cycle deregulation, and DNA fragmentation. Moreover, co-treatment led to GSH depletion showing its pro-apoptotic effect dependent on ROS overproduction. The treatment did not show a significant effect on normal cells-melanocytes-which indicates its high selectivity. The results suggest a possible benefit from the use of the ketoprofen and ultraviolet A irradiation as a new concept of melanotic melanoma therapy.
Authors	Klaudia Banach, Justyna Kowalska, Zuzanna Rzepka, Artur Beberok, Jakub Rok, Dorota Wrześniok
Journal	International journal of molecular sciences (Int J Mol Sci) Vol. 22 Issue 21 (Nov 04 2021) ISSN: 1422-0067 [Electronic] Switzerland
PMID	34769396 (Publication Type: Journal Article)
Chemical References	Anti-Inflammatory Agents, Non-Steroidal Ketoprofen
Topics	Anti-Inflammatory Agents, Non-Steroidal (pharmacology) Apoptosis Cell Proliferation Cells, Cultured Chemoradiotherapy Humans Ketoprofen (pharmacology) Melanocytes (drug effects, pathology, radiation effects) Melanoma (drug therapy, pathology, radiotherapy) Ultraviolet Rays

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