Molecular surveillance and genetic divergence of rotavirus A antigenic epitopes in Gabonese children with acute gastroenteritis.

Abstract

BACKGROUND:

Rotavirus A (RVA) causes acute gastroenteritis in children <5 years of age in sub-Saharan Africa. In this study, we described the epidemiology and genetic diversity of RVA infecting Gabonese children and examined the antigenic variability of circulating strains in relation to available vaccine strains to maximize the public health benefits of introducing rotavirus vaccine through the Expanded Programme on Immunization (EPI) in Gabon.

METHODS:

Stool samples were collected consecutively between April 2018 and November 2019 from all hospitalized children <5 years with gastroenteritis and community controls without gastroenteritis. Children were tested for rotavirus A by quantitative RT-PCR and subsequently sequenced to identify circulating rotavirus A genotypes in the most vulnerable population. The VP7 and VP4 (VP8*) antigenic epitopes were mapped to homologs of vaccine strains to assess structural variability and potential impact on antigenicity.

FINDINGS:

Infections were mostly acquired during the dry season. Rotavirus A was detected in 98/177 (55%) hospitalized children with gastroenteritis and 14/67 (21%) of the control children. The most common RVA genotypes were G1 (18%), G3 (12%), G8 (18%), G9 (2%), G12 (25%), with G8 and G9 reported for the first time in Gabon. All were associated either with P[6] (31%) or P[8] (38%) genotypes. Several non-synonymous substitutions were observed in the antigenic epitopes of VP7 (positions 94 and 147) and VP8* (positions 89, 116, 146 and 150), which may modulate the elicited immune responses.

INTERPRETATION:

This study contributes to the epidemiological surveillance of rotavirus A required before the introduction of rotavirus vaccination in the EPI for Gabonese children.

• Authors: Gédéon Prince Manouana, Sandra Niendorf, Alexandru Tomazatos, Mirabeau Mbong Ngwese, Moustapha Nzamba Maloum, Paul Alvyn Nguema Moure, Gedeon Bingoulou Matsougou, Simon Ategbo, Elie Gide Rossatanga, C Thomas Bock, Steffen Borrmann, Benjamin Mordmüller, Daniel Eibach, Peter G Kremsner, Thirumalaisamy P Velavan, Ayola Akim Adegnika
• Journal: EBioMedicine (EBioMedicine) Vol. 73 Pg. 103648 (Nov 2021) ISSN: 2352-3964 [Electronic] Netherlands
• PMID: 34706308 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.

Chemical References

• Antigens, Viral
• Epitopes

Topics

• Amino Acid Sequence
• Antigenic Variation
• Antigens, Viral (chemistry, genetics, immunology)
• Child, Preschool
• Epitopes (chemistry, genetics, immunology)
• Female
• Gabon (epidemiology)
• Gastroenteritis (epidemiology, virology)
• Genetic Variation
• Genotype
• Humans
• Infant
• Infant, Newborn
• Male
• Molecular Epidemiology
• Phylogeny
• Prevalence
• Public Health Surveillance
• Rotavirus (classification, genetics)
• Rotavirus Infections (epidemiology, virology)
• Seasons