Faecal Microbiota
Transplantation (FMT) is considered as a promising technology to fight against
obesity. Wild boar has leanermuscle and less fat in comparison to the domestic pig, which were thought to be related with microbiota. To investigate the function and mechanism of the wild boar microbiota on
obesity, we first analysed the wild boar microbiota composition via 16S
rDNA sequencing, which showed that Firmicutes and Proteobacteria were the dominant bacteria. Then, we established a high-fat diet (HFD)-induced
obesity model, and transfer low and high concentrations of wild boar faecal
suspension in mice for 9 weeks. The results showed that FMT prevented HFD-induced
obesity and
lipid metabolism disorders, and altered the jejunal microbiota composition especially increasing the abundance of the Lactobacillus and Romboutsia, which were negatively correlated with
obesity-related indicators. Moreover, we found that the anti-
obesity effect of wild boar faecal
suspension was associated with jejunal
N6-methyladenosine (m6 A) levels. Overall, these results suggest that FMT has a mitigating effect on HFD-induced
obesity, which may be due to the impressive effects of FMT on the microbial composition and structure of the jejunum. These changes further alter intestinal lipid metabolism and m6 A levels to achieve resistance to
obesity.