Abstract | BACKGROUND/AIM: PATIENTS AND METHODS: PC and PA were taken from a 63-year-old patient. Using two-dimensional differential gel electrophoresis (2D-DIGE) coupled to mass spectrometry we examined the differences between PC and PA proteins. For validation, additional PC and PA samples were obtained from 10 patients. Western blot analysis was used to validate the difference of expression observed with 2D-DIGE analysis. Bioinfomatic analysis was performed using QIAGEN's Ingenuity Pathways Analysis (IPA) to determine the predominant canonical pathways and interaction networks involved. RESULTS: Thirty-three differentially expressed proteins were identified in PC compared to PA. Among these, ubiquitin C-terminal hydrolase-L1 (UCH-L1) was highly overexpressed in PC. The result was confirmed by Western Blot analysis in additional PC samples. CONCLUSION: Our comparative proteomic analysis of co-existing neoplasms allowed detecting specific and peculiar differences between PC and PA overcoming population biological variability.
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Authors | Federica Ciregia, Filomena Cetani, Elena Pardi, Alessio Soggiu, Cristian Piras, Lorenzo Zallocco, Simona Borsari, Maurizio Ronci, Vanni Caruso, Claudio Marcocci, Maria Rosa Mazzoni, Antonio Lucacchini, Laura Giusti |
Journal | Cancer genomics & proteomics
(Cancer Genomics Proteomics)
2021 Nov-Dec
Vol. 18
Issue 6
Pg. 781-796
ISSN: 1790-6245 [Electronic] Greece |
PMID | 34697069
(Publication Type: Case Reports, Journal Article)
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Copyright | Copyright© 2021, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. |
Topics |
- Adenoma
(diagnosis, pathology)
- Humans
- Male
- Middle Aged
- Parathyroid Neoplasms
(diagnosis, pathology)
- Proteomics
(methods)
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