Abstract |
Cisplatin (CDDP) is widely used for chemotherapy of esophageal squamous cell carcinoma (ESCC) but the drug resistance limits its therapeutic benefit. Heterogeneous nuclear ribonucleoprotein U-like 1 (HNRNPUL1) belongs to the family of RNA-binding proteins (RBPs) and is involved in DNA damage repair. To investigate whether and how HNRNPUL1 affects CDDP resistance of ESCC, we evaluated the expression of HNRNPUL1 and found that it was associated with recurrence in ESCC patients receiving postoperative platinum-based chemotherapy and was an independent prognostic factor for disease-free survival (DFS). Besides, we showed that the reduced expression of HNRNPUL1 enhanced the CDDP sensitivity of ESCC cells. Furthermore, RNA immunoprecipitation coupled with high-throughput sequencing (RIP-seq) were performed and a range of HNRNPUL1-binding RNAs influenced by CDDP treatment were identified followed by bioinformatics analysis. In terms of mechanism, we found that HNRNPUL1 inhibited CDDP sensitivity of ESCC cells by regulating the CDDP sensitivity-inhibited circular RNA ( circRNA) MAN1A2 formation. Taken together, our results first demonstrated the role of HNRNPUL1 in CDDP resistance of ESCC and suggested that HNRNPUL1 may be a potential target of ESCC chemotherapy.
|
Authors | Juan Li, Meixiang Sang, Yang Zheng, Lingjiao Meng, Lina Gu, Ziyi Li, Fei Liu, Yunyan Wu, Weijing Li, Baoen Shan |
Journal | Experimental cell research
(Exp Cell Res)
Vol. 409
Issue 1
Pg. 112891
(12 01 2021)
ISSN: 1090-2422 [Electronic] United States |
PMID | 34688610
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2021 Elsevier Inc. All rights reserved. |
Chemical References |
- HNRNPUL1 protein, human
- Heterogeneous-Nuclear Ribonucleoproteins
- Nuclear Proteins
- RNA, Circular
- Transcription Factors
- Cisplatin
|
Topics |
- Animals
- Apoptosis
(drug effects, genetics)
- Cell Line, Tumor
- Cell Proliferation
(drug effects, genetics)
- Cisplatin
(therapeutic use)
- Drug Resistance, Neoplasm
(drug effects, genetics)
- Esophageal Neoplasms
(drug therapy, genetics)
- Esophageal Squamous Cell Carcinoma
(drug therapy, genetics)
- Female
- Gene Expression Regulation, Neoplastic
(drug effects, genetics)
- Heterogeneous-Nuclear Ribonucleoproteins
(genetics)
- Humans
- Male
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Middle Aged
- Nuclear Proteins
(genetics)
- RNA, Circular
(genetics)
- Transcription Factors
(genetics)
|