Aptamer Embedded Arch-Cruciform DNA Assemblies on 2-D VS2 Scaffolds for Sensitive Detection of Breast Cancer Cells.

Abstract

Arch-cruciform DNA are self-assembled on AuNPs/VS2 scaffold as a highly sensitive and selective electrochemical biosensor for michigan cancer foundation-7 (MCF-7) breast cancer cells. In the construction, arch DNA is formed using two single-strand DNA sequences embedded with the aptamer for MCF-7 cells. In the absence of MCF-7 cells, a cruciform DNA labeled with three terminal biotin is bound to the top of arch DNA, which further combines with streptavidin-labeled horseradish peroxidase (HRP) to catalyze the hydroquinone-H2O2 reaction on the electrode surface. The presence of MCF-7 cells can release the cruciform DNA and reduce the amount of immobilized HRP, thus effectively inhibiting enzyme-mediated electrocatalysis. The electrochemical response of the sensor is negatively correlated with the concentration of MCF-7 cells, with a linear range of 10~1 × 105 cells/mL, and a limit of detection as low as 5 cells/mL (S/N = 3). Through two-dimensional materials and enzyme-based dual signal amplification, this biosensor may pave new ways for the highly sensitive detection of tumor cells in real samples.

• Authors: Jinfeng Quan, Yihan Wang, Jialei Zhang, Kejing Huang, Xuemei Wang, Hui Jiang
• Journal: Biosensors (Biosensors (Basel)) Vol. 11 Issue 10 (Oct 08 2021) ISSN: 2079-6374 [Electronic] Switzerland
• PMID: 34677334 (Publication Type: Journal Article)

Chemical References

• Aptamers, Nucleotide
• DNA, Cruciform
• Gold
• Hydrogen Peroxide
• Horseradish Peroxidase

Topics

• Aptamers, Nucleotide
• Biosensing Techniques
• Breast Neoplasms
• DNA, Cruciform
• Electrochemical Techniques
• Female
• Gold
• Horseradish Peroxidase (metabolism)
• Humans
• Hydrogen Peroxide
• Limit of Detection
• Metal Nanoparticles