Abstract | OBJECTIVES:
Feline infectious peritonitis (FIP), caused by genetic mutants of feline enteric coronavirus known as FIPV, is a highly fatal disease of cats with no currently available vaccine or US Food and Drug Administration-approved cure. Dissemination of FIPV in affected cats results in a range of clinical signs, including cavitary effusions, anorexia, fever and lesions of pyogranulomatous vasculitis and perivasculitis, with or without central nervous system or ocular involvement. The objectives of this study were to screen an array of antiviral compounds for anti-FIPV (serotype II) activity, determine cytotoxicity safety profiles of identified compounds with anti-FIPV activity and strategically combine identified monotherapies to assess compound synergy against FIPV in vitro. Based upon clinically successful combination treatment strategies for human patients with HIV and hepatitis C virus infections, we hypothesized that a combined anticoronaviral therapy approach featuring concurrent multiple mechanisms of drug action would result in an additive or synergistic antiviral effect. METHODS: This study screened 90 putative antiviral compounds for efficacy and cytotoxicity using a multimodal in vitro strategy, including plaque bioassays, real-time RT-PCR viral inhibition and cytotoxicity assays. RESULTS: CONCLUSIONS AND RELEVANCE: Although evidence of compound synergy was identified for several combinations of antiviral agents, monotherapies were ultimately determined to be the most effective in the inhibition of viral transcription.
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Authors | Sarah E Cook, Helena Vogel, Diego Castillo, Mark Olsen, Niels Pedersen, Brian G Murphy |
Journal | Journal of feline medicine and surgery
(J Feline Med Surg)
Vol. 24
Issue 10
Pg. 943-953
(10 2022)
ISSN: 1532-2750 [Electronic] England |
PMID | 34676775
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antiviral Agents
- Drug Combinations
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Topics |
- Animals
- Antiviral Agents
(pharmacology, therapeutic use)
- Cat Diseases
(drug therapy)
- Cats
- Coronavirus, Feline
(genetics)
- Drug Combinations
- Feline Infectious Peritonitis
- Humans
- Serogroup
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