Gastric cancer (GC) is the most common gastrointestinal
malignancy worldwide. However, the molecular mechanisms of the progression of GC are not fully understood. Ras-responsive
element binding protein 1 (RREB1) is an oncogene in many types of
cancer that is involved in various biological processes, such as DNA damage repair, cell growth and proliferation, cell differentiation, fat development, and fasting
glucose balance. In this study, we demonstrate the role of RREB1 in
gastric cancer. First, by immunohistochemistry staining (IHC) and bioinformatics analysis, we demonstrated the expression of RREB1 in
gastric cancer and paired normal gastric tissues. Then, we established RREB1 overexpression and knockdown cell lines via lentiviral transfection and detected cell proliferation by using MTT, colony-forming, cell cycle and apoptosis assays in vitro. We demonstrated the effect of RREB1 on cell proliferation in vivo by using a subcutaneous xenograft
tumor model in nude mice. Finally, by using Western blotting and IHC, we demonstrated the possible mechanism by which RREB1 affects cell proliferation. The IHC and bioinformatics analyses demonstrated that RREB1 was highly expressed in
gastric cancer and showed that RREB1-expressing patients had a larger
tumor size and more lymphovascular invasion than RREB1-negative patients. Knockdown of RREB1 inhibited cell proliferation in vivo and in vitro. Knockdown of RREB1 enhanced p16 expression in vivo and in vitro, and p16 expression was negatively related to RREB1 in
gastric cancer tissue. RREB1 was highly expressed in
gastric cancer, and knockdown of RREB1 inhibited cell proliferation via enhanced p16 expression.