A Portable Digital Loop-Mediated Isothermal Amplification Platform Based on Microgel Array and Hand-Held Reader.

Abstract

Digital polymerase chain reaction (dPCR) has found widespread applications in molecular diagnosis of various diseases owing to its sensitive single-molecule detection capability. However, the existing dPCR platforms rely on the auxiliary procedure to disperse DNA samples, which needs complicated operation, expensive apparatus, and consumables. Besides, the complex and costly dPCR readers also impede the applications of dPCR for point-of-care testing (POCT). Herein, we developed a portable digital loop-mediated isothermal amplification (dLAMP) platform, integrating a microscale hydrogel (microgel) array chip for sample partition, a miniaturized heater for DNA amplification, and a hand-held reader for digital readout. In the platform, the chip with thousands of isolated microgels holds the capability of self-absorption and partition of DNA samples, thus avoiding auxiliary equipment and professional personnel operations. Using the integrated dLAMP platform, λDNA templates have been quantified with a good linear detection range of 2-1000 copies/μL and a detection limit of 1 copy/μL. As a demonstration, the epidermal growth factor receptor L858R gene mutation, a crucial factor for the susceptibility of the tyrosine kinase inhibitor in non-small-cell lung cancer treatment, has been accurately identified by the dLAMP platform with a spiked plasma sample. This work shows that the developed dLAMP platform provides a low-cost, facile, and user-friendly solution for the absolute quantification of DNA, showing great potential for the POCT of nucleic acids.

• Authors: Lei Cao, Xiaojin Guo, Ping Mao, Yulin Ren, Zedong Li, Minli You, Jie Hu, Miao Tian, Chunyan Yao, Fei Li, Feng Xu
• Journal: ACS sensors (ACS Sens) Vol. 6 Issue 10 Pg. 3564-3574 (10 22 2021) ISSN: 2379-3694 [Electronic] United States
• PMID: 34606243 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Microgels

Topics

• Carcinoma, Non-Small-Cell Lung
• Humans
• Lung Neoplasms
• Microgels
• Molecular Diagnostic Techniques
• Nucleic Acid Amplification Techniques