Abstract |
Inflammation and fibrosis are intertwined mechanisms fundamentally involved in heart failure. Detailed deciphering gene expression perturbations and cell-cell interactions of leukocytes and non-myocytes is required to understand cell-type-specific pathology in the failing human myocardium. To this end, we performed single-cell RNA sequencing and single T cell receptor sequencing of 200,615 cells in both human dilated cardiomyopathy (DCM) and ischemic cardiomyopathy (ICM) hearts. We sampled both lesion and mild-lesion tissues from each heart to sequentially capture cellular and molecular alterations to different extents of cardiac fibrosis. By which, left (lesion) and right ventricle (mild-lesion) for DCM hearts were harvest while infarcted (lesion) and non-infarcted area (mild-lesion) were dissected from ICM hearts. A novel transcription factor AEBP1 was identified as a crucial cardiac fibrosis regulator in ACTA2+ myofibroblasts. Within fibrotic myocardium, an infiltration of a considerable number of leukocytes was witnessed, especially cytotoxic and exhausted CD8+ T cells and pro-inflammatory CD4+ T cells. Furthermore, a subset of tissue-resident macrophage, CXCL8hiCCR2+HLA-DRhi macrophage was particularly identified in severely fibrotic area, which interacted with activated endothelial cell via DARC, that potentially facilitate leukocyte recruitment and infiltration in human heart failure.
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Authors | Man Rao, Xiliang Wang, Guangran Guo, Li Wang, Shi Chen, Pengbin Yin, Kai Chen, Liang Chen, Zemin Zhang, Xiao Chen, Xueda Hu, Shengshou Hu, Jiangping Song |
Journal | Basic research in cardiology
(Basic Res Cardiol)
Vol. 116
Issue 1
Pg. 55
(10 03 2021)
ISSN: 1435-1803 [Electronic] Germany |
PMID | 34601654
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2021. Springer-Verlag GmbH Germany, part of Springer Nature. |
Chemical References |
- AEBP1 protein, human
- Repressor Proteins
- Carboxypeptidases
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Topics |
- CD8-Positive T-Lymphocytes
- Carboxypeptidases
- Cardiomyopathy, Dilated
(genetics, pathology)
- Fibrosis
- Heart Failure
(pathology)
- Humans
- Inflammation
(pathology)
- Myocardium
(pathology)
- Repressor Proteins
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