miR-34a Suppresses Cell Proliferation in Laryngeal Cancer by Targeting Prominin 1.

Abstract

The study explored the cross-talk between the microRNA miR-34a and prominin 1 in the development of laryngeal cancer (LC), which has an unacceptable high mortality rate. We predicted that miR-34a might target prominin 1. miR-34a and prominin 1 expression was analyzed with reverse transcription quantitative polymerase chain reaction. The correlation between miR-34a and prominin 1 was determined by linear regression analysis. miR-34a and prominin 1 overexpression and miR-34a inhibition were achieved in LC cells to analyze their relationship. The roles of miR-34a and prominin 1 in LC cell proliferation were studied with CCK-8 assay. The results showed miR-34a was downregulated, and prominin 1 was upregulated in LC. In addition, prominin 1 and miR-34a were inversely correlated across cancer tissue samples. In cancer cells, miR-34a overexpression downregulated prominin 1, while miR-34a knockdown upregulated prominin 1. Moreover, miR-34a overexpression reduced the enchaining effects of prominin 1 on LC cell proliferation. Therefore, miR-34a might suppress LC cell proliferation by targeting prominin 1.

• Authors: Xianyang Luo, Aimin Chen, Yi Zhou, Binghuang Zhang, Yuanqin Jiang, Zhizong Hong
• Journal: Critical reviews in eukaryotic gene expression (Crit Rev Eukaryot Gene Expr) Vol. 31 Issue 5 Pg. 51-57 ( 2021) ISSN: 1045-4403 [Print] United States
• PMID: 34591390 (Publication Type: Journal Article)

Chemical References

• AC133 Antigen
• MIRN34 microRNA, human
• MicroRNAs
• PROM1 protein, human

Topics

• AC133 Antigen (metabolism)
• Aged
• Apoptosis
• Cell Line, Tumor
• Cell Movement
• Cell Proliferation
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Laryngeal Neoplasms (genetics, metabolism)
• Male
• MicroRNAs (metabolism)
• Middle Aged
• Transcriptome