In Silico Study, Physicochemical, and In Vitro Lipase Inhibitory Activity of α,β-Amyrenone Inclusion Complexes with Cyclodextrins.

Abstract

α,β-amyrenone (ABAME) is a triterpene derivative with many biological activities; however, its potential pharmacological use is hindered by its low solubility in water. In this context, the present work aimed to develop inclusion complexes (ICs) of ABAME with γ- and β-cyclodextrins (CD), which were systematically characterized through molecular modeling studies as well as FTIR, XRD, DSC, TGA, and SEM analyses. In vitro analyses of lipase activity were performed to evaluate possible anti-obesity properties. Molecular modeling studies indicated that the CD:ABAME ICs prepared at a 2:1 molar ratio would be more stable to the complexation process than those prepared at a 1:1 molar ratio. The physicochemical characterization showed strong evidence that corroborates with the in silico results, and the formation of ICs with CD was capable of inducing changes in ABAME physicochemical properties. ICs was shown to be a stronger inhibitor of lipase activity than Orlistat and to potentiate the inhibitory effects of ABAME on porcine pancreatic enzymes. In conclusion, a new pharmaceutical preparation with potentially improved physicochemical characteristics and inhibitory activity toward lipases was developed in this study, which could prove to be a promising ingredient for future formulations.

• Authors: Luana Carvalho de Oliveira, Danielle Lima Bezerra de Menezes, Valéria Costa da Silva, Estela Mariana Guimarães Lourenço, Paulo Henrique Santana Miranda, Márcia de Jesus Amazonas da Silva, Emerson Silva Lima, Valdir Florêncio da Veiga Júnior, Ricardo Neves Marreto, Attilio Converti, Euzébio Guimaraes Barbosa, Ádley Antonini Neves de Lima
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 22 Issue 18 (Sep 13 2021) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 34576044 (Publication Type: Journal Article)

Chemical References

• Enzyme Inhibitors
• Triterpenes
• beta-Cyclodextrins
• alpha-amyrenone
• Orlistat
• Lipase

Topics

• Animals
• Calorimetry, Differential Scanning
• Computer Simulation
• Enzyme Inhibitors (chemistry, pharmacology)
• Lipase (antagonists & inhibitors, chemistry)
• Orlistat (pharmacology)
• Solubility (drug effects)
• Spectroscopy, Fourier Transform Infrared
• Swine
• Triterpenes (chemical synthesis, chemistry, pharmacology)
• X-Ray Diffraction
• beta-Cyclodextrins (chemistry, pharmacology)