Involvement of RFamide neuropeptides in polyp contraction of the adult scleractinian corals Euphyllia ancora and Stylophora pistillata.

Abstract

The distribution and functions of neurons in scleractinian corals remain largely unknown. This study focused on the Arg-Phe amide family of neuropeptides (RFamides), which have been shown to be involved in a variety of biological processes in animals, and performed molecular identification and characterization in the adult scleractinian coral Euphyllia ancora. The deduced amino acid sequence of the identified RFamide preprohormone was predicted to contain 20 potential neuropeptides, including 1 Pro-Gly-Arg-Phe (PGRF) amide and 15 Gln-Gly-Arg-Phe (QGRF) amide peptides. Tissue distribution analysis showed that the level of transcripts in the tentacles was significantly higher than that in other polyp tissues. Immunohistochemical analysis with the FMRFamide antibody showed that RFamide neurons were mainly distributed in the epidermis of the tentacles and mouth with pharynx. Treatment of E. ancora polyps with synthetic QGRFamide peptides induced polyp contraction. The induction of polyp contraction by QGRFamide peptide treatment was also observed in another scleractinian coral, Stylophora pistillata. These results strongly suggested that RFamides play a role in the regulation of polyp contraction in adult scleractinians.

• Authors: Yan Zhang, Shinya Shikina, Yu-Ying Ho, Yi-Ling Chiu, Jack I-Chen Yao, Céline Zatylny-Gaudin, Sylvie Dufour, Ching-Fong Chang
• Journal: General and comparative endocrinology (Gen Comp Endocrinol) Vol. 314 Pg. 113905 (12 01 2021) ISSN: 1095-6840 [Electronic] United States
• PMID: 34534544 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021. Published by Elsevier Inc.

Chemical References

• Neuropeptides
• arginylphenylalaninamide
• FMRFamide

Topics

• Amino Acid Sequence
• Animals
• Anthozoa
• FMRFamide
• Neuropeptides (metabolism)