Abstract | BACKGROUND: METHODS: We used a short-term single dose of cisplatin to induce the dormant and reactivated cell status. We examined the gene expressional profiling and proteomic profiling of EVPs from dormant and reactivated cancer cells by RNA-sequencing and LC-MS/MS. RESULTS: We found substantial changes in gene expression and protein level in EVP. The genes with higher expression in dormant cancer cells were lipid transporter- and lipid metabolic-related genes. A total of 111 EVP proteins were upregulated in dormant cancer cells compared to those in control cells. Fifty differential expressed proteins (DEPs) were identified in EVPs from reactivated cancer cells compared to those in dormant cancer cells. Among the DEPs, we found that apolipoproteins such as APOA1 and APOE were significantly increased in dormant cancer cell-derived EVPs. Integration of EVP proteomes with transcriptional profiles of cancer cells revealed that the proteomic profiling of EVP derived from cancer cells can reflect the cellular status of cancer cells, which showed an activated lipid metabolism in dormant state. CONCLUSION:
|
Authors | Jiaqi Xu, Lujuan Wang, Na Yin, Anqi Chen, Junqi Yi, Jingqun Tang, Juanjuan Xiang |
Journal | Thoracic cancer
(Thorac Cancer)
Vol. 12
Issue 19
Pg. 2601-2610
(10 2021)
ISSN: 1759-7714 [Electronic] Singapore |
PMID | 34520129
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | © 2021 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd. |
Chemical References |
- Antineoplastic Agents
- Cisplatin
|
Topics |
- A549 Cells
- Antineoplastic Agents
(pharmacology)
- Carcinoma, Non-Small-Cell Lung
(drug therapy)
- Cisplatin
(pharmacology)
- Extracellular Vesicles
(drug effects)
- Humans
- Lung Neoplasms
(drug therapy)
- Protein Interaction Maps
- Proteomics
|