Arginine-Arginine-Leucine Peptide Targeting Heat Shock Protein 70 for Cancer Imaging.

Abstract

Arg-Arg-Leu (RRL) is a potent tumor-homing tripeptide. However, the binding target is unclear. In this study, we intended to identify the binding target of RRL and evaluate the tumor targeting of 99mTc-MAG3-RRL in vivo. Biotin-RRL, 5-TAMRA-RRL, and 99mTc-MAG3-RRL were designed to trace the binding target and tumor lesion. Immunoprecipitation-mass spectrometry was conducted to identify the candidate proteins and determination of the subcellular localization was also performed. A pull-down assay was performed to demonstrate the immunoprecipitate. Fluorescence colocalization and cell uptake assays were performed to elucidate the correlation between the selected binding protein and RRL, and the internalization mechanism of RRL. Biodistribution and in vivo imaging were performed to evaluate the tumor accumulation and targeting of 99mTc-MAG3-RRL. The target for RRL was screened to be heat shock protein 70 (HSP70). The prominent uptake distribution of RRL was concentrated in the membrane and cytoplasm. A pull-down assay demonstrated the existence of HSP70 in the biotin-RRL captured complex. Regarding fluorescence colocalization and cell uptake assays, RRL may interact with HSP70 at the nucleotide-binding domain (NBD). Clathrin-dependent endocytosis and macropinocytosis could be a vital internalization mechanism of RRL. In vivo imaging and biodistribution both demonstrated that 99mTc-MAG3-RRL can trace tumors with satisfactory accumulation in hepatoma xenograft mice. The radioactive signals accumulated in tumor lesions can be blocked by VER-155008, which can bind to the NBD of HSP70. Our findings revealed that RRL may interact with HSP70 and that 99mTc-MAG3-RRL could be a prospective probe for visualizing overexpressed HSP70 tumor sections.

• Authors: Yujing Du, Zhao Chen, Ping Yan, Chunli Zhang, Xiaojiang Duan, Xueqi Chen, Meng Liu, Lei Kang, Xing Yang, Yan Fan, Jianhua Zhang, Rongfu Wang
• Journal: Molecular pharmaceutics (Mol Pharm) Vol. 18 Issue 10 Pg. 3750-3762 (10 04 2021) ISSN: 1543-8392 [Electronic] United States
• PMID: 34491767 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• HSP70 Heat-Shock Proteins
• Oligopeptides
• arginyl-arginyl-leucine

Topics

• Animals
• Binding Sites
• Female
• Flow Cytometry
• HSP70 Heat-Shock Proteins (metabolism)
• HeLa Cells (metabolism)
• Hep G2 Cells (metabolism)
• Humans
• Immunoprecipitation
• MCF-7 Cells (metabolism)
• Mass Spectrometry
• Mice
• Mice, Nude
• Neoplasms (diagnostic imaging)
• Oligopeptides (metabolism)
• Optical Imaging
• Tomography, Emission-Computed, Single-Photon