Simplified, Readily Available Method for the Treatment of Band Keratopathy With Ethylenediaminetetraacetic Acid.

Abstract

PURPOSE:

To compare 3 methods for creating ethylenediaminetetraacetic acid (EDTA) solution using readily available Vacutainer tubes for the treatment of band keratopathy.

METHODS:

All 3 protocols used commercially available Vacutainer blood collection tubes coated with K2EDTA. An osmometer was used to measure and compare the concentration of EDTA created using 3 different protocols. The time required for preparation of the solution was measured and compared to evaluate its efficiency for everyday clinical use. In addition, volume of EDTA solution obtained was measured for method 1. The most promising protocol for clinical use was then used for treatment of a series of patients.

RESULTS:

Average osmolarity was 532, 285, and 422 for methods 1, 2, and 3, respectively (ANOVA P < 0.01, all Tukey honestly significant difference P < 0.01). For the respective mixtures, average concentration was 65, 35, and 52 mg/mL, and average time to create solution was 189, 38, and 83 seconds (ANOVA P < 0.01, all Tukey honestly significant difference P < 0.01). The most promising, method 3, was found to be safe and effective in removing calcium from the corneal stroma in a series of 5 patients with 6 eyes treated. It also yielded 25% more solution for clinical use than method 1.

CONCLUSIONS:

Method 3 using a single 10-mL Vacutainer tube with 18 mg of K2-EDTA had the best balance of effective concentration of EDTA, time to preparation, and simplicity of methodology, when compared with previously published methods 1 and 2. It also yielded a greater final volume of solution.

• Authors: Julio Narvaez, Matthew Chang, Jeffrey Ing, Daniel De Chance, Joshua J Narvaez
• Journal: Cornea (Cornea) Vol. 40 Issue 10 Pg. 1360-1362 (Oct 01 2021) ISSN: 1536-4798 [Electronic] United States
• PMID: 34481414 (Publication Type: Case Reports, Comparative Study, Journal Article)
• Copyright: Copyright © 2020 The Author(s). Published by Wolters Kluwer Health, Inc.

Chemical References

• Calcium Chelating Agents
• Ophthalmic Solutions
• Pharmaceutical Preparations
• Edetic Acid

Topics

• Administration, Ophthalmic
• Aged
• Aged, 80 and over
• Calcium Chelating Agents (therapeutic use)
• Corneal Dystrophies, Hereditary (drug therapy, physiopathology)
• Edetic Acid (therapeutic use)
• Female
• Humans
• Male
• Middle Aged
• Ophthalmic Solutions
• Osmolar Concentration
• Pharmaceutical Preparations
• Visual Acuity (physiology)