Abstract | BACKGROUND:
Irritable bowel syndrome (IBS) patients often experience meal-associated symptoms. However, the underlying mechanisms are unclear. AIM: To determine small intestinal mechanisms of lipid-induced symptoms and rectal hypersensitivity in IBS METHODS: We recruited 26 IBS patients (12 IBS-C, 14 IBS-D) and 15 healthy volunteers (HV). In vivo permeability was assessed using saccharide excretion assay. Rectal sensitivity was assessed using a barostat before and after small bowel lipid infusion; symptoms were assessed throughout. Next, an extended upper endoscopy with probe-based confocal laser endomicroscopy (pCLE) was performed with changes induced by lipids. Duodenal and jejunal mucosal biopsies were obtained for transcriptomics. RESULTS: Following lipid infusion, a higher proportion of HV than IBS patients reported no pain, no nausea, no fullness and no urgency (P < 0.05 for all). In a model adjusted for sex and anxiety, IBS-C and IBS-D patients had lower thresholds for first rectal sensation (P = 0.0007) and pain (P = 0.004) than HV. In vivo small intestinal permeability and mean pCLE scores were similar between IBS patients and HV. Post- lipid, pCLE scores were higher than pre- lipid but were not different between groups. Baseline duodenal transient receptor potential vanilloid (TRPV) 1 and 3 expression was increased in IBS-D, and TRPV3 in IBS-C. Duodenal TRPV1 expression correlated with abdominal pain (r = 0.51, FDR = 0.01), and inversely with first rectal sensation (r = -0.48, FDR = 0.01) and pain (r = -0.41, FDR = 0.02) thresholds. CONCLUSION:
Lipid infusion elicits a greater symptom response in IBS patients than HV, which is associated with small intestinal expression of TRPV channels. TRPV-mediated small intestinal chemosensitivity may mediate post-meal symptoms in IBS.
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Authors | Madhusudan Grover, Antonio Berumen, Stephanie Peters, Ting Wei, Margaret Breen-Lyles, William S Harmsen, Irene Busciglio, Duane Burton, Maria Vazquez Roque, Kenneth R DeVault, Michael Camilleri, Michael Wallace, Surendra Dasari, Helmut Neumann, Lesley A Houghton |
Journal | Alimentary pharmacology & therapeutics
(Aliment Pharmacol Ther)
Vol. 54
Issue 9
Pg. 1179-1192
(11 2021)
ISSN: 1365-2036 [Electronic] England |
PMID | 34472640
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2021 John Wiley & Sons Ltd. |
Chemical References |
- Transient Receptor Potential Channels
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Topics |
- Abdominal Pain
- Humans
- Intestine, Small
- Irritable Bowel Syndrome
(drug therapy)
- Rectum
- Transient Receptor Potential Channels
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