A single low-energy shockwave pulse opens blood-cerebrospinal fluid barriers and facilitates gastrodin delivery to alleviate epilepsy.
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| Abstract |
The blood-cerebrospinal fluid barrier (BCSFB) is another gatekeeper between systemic circulation and the central nervous system (CNS), mainly present at the boundary between choroid plexuses and the ventricular system. This study demonstrates BCSFB opening in rats by single pulse of low-energy focused shockwave (FSW, energy flux density 0.03 mJ/mm2, 2 × 106 microbubbles/kg) treatment at lateral ventricle, resulting in significantly elevated cerebrospinal fluid (CSF) concentrations of systemically-administered gastrodin (GTD) (4 times vs. control within 3 hrs) that remained detectable for 24 hrs. The FSW-GTD group had significantly lower Racine's scale (<4) and zero mortality (n = 30) after lithium-pilocarpine-induced epilepsy. Electrophysiological recordings showed decreased epileptiform discharges, and brain section histology revealed reduced inflammation, oxidative stress and apoptosis, when compared with groups without FSW (Racine's scale: 4 ∼ 5; mortality: 26.67 ∼ 36.67%). FSW-mediated BCSFB opening provides a promising alternative for controlled-delivery of therapeutics into the CNS, offering rapid and widespread medication distribution. The technique could by applied in the development of novel therapies for various CNS diseases.
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| Authors | Yi Kung, Ming-Yen Hsiao, Shu-Mei Yang, Tz-Yi Wen, Moxian Chen, Wei-Hao Liao, Chueh-Hung Wu, Lijuan Ao, Wen-Shiang Chen |
| Journal | Ultrasonics sonochemistry (Ultrason Sonochem) Vol. 78 Pg. 105730 (Oct 2021) ISSN: 1873-2828 [Electronic] Netherlands |
| PMID | 34464899 (Publication Type: Journal Article) |
| Copyright | Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved. |
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