Abstract | BACKGROUND:
Eosinophilic esophagitis (EoE) is a food allergen driven disease that is accompanied by interleukin ( IL) 13 overexpression and esophageal barrier dysfunction allowing transepithelial food allergen permeation. Nutraceuticals, such as short-chain fatty acids (SCFAs) that restore barrier function and increase immune fitness may be a promising tool in the management of EoE. Here, we investigated the effects of the SCFAs acetate, propionate, and butyrate on an IL-13-compromised human esophageal epithelial barrier, including the mechanisms involved. METHODS: An air-liquid interface culture model of differentiated human EPC2-hTERT (EPC2) was used to study whether SCFAs could restore barrier function after IL-13-induced impairment. Esophageal epithelial barrier function was monitored by transepithelial electrical resistance (TEER) and FITC-dextran paracellular flux, and was further examined by qPCR and immunohistochemical analysis. G protein-coupled receptor (GPR) GPR41, GPR43, GPR109a, or histone deacetylase (HDAC) (ant)agonists were used to assess mechanisms of action of SCFAs. RESULTS: CONCLUSION: Nutraceuticals butyrate and propionate restore the barrier function of esophageal epithelial cells after an inflammatory insult and may be of therapeutic benefit in the management of EoE.
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Authors | Mirelle T A Kleuskens, Maria L Haasnoot, Bart M Herpers, Marleen T J van Ampting, Albert J Bredenoord, Johan Garssen, Frank A Redegeld, Betty C A M van Esch |
Journal | Allergy
(Allergy)
Vol. 77
Issue 5
Pg. 1510-1521
(05 2022)
ISSN: 1398-9995 [Electronic] Denmark |
PMID | 34458999
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2021 The Authors. Allergy published by European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd. |
Chemical References |
- Allergens
- Butyrates
- Fatty Acids, Volatile
- Interleukin-13
- Propionates
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Topics |
- Allergens
(therapeutic use)
- Butyrates
(pharmacology, therapeutic use)
- Eosinophilic Esophagitis
(drug therapy)
- Fatty Acids, Volatile
(pharmacology)
- Humans
- Interleukin-13
(metabolism)
- Propionates
(pharmacology)
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