LncRNA DLGAP1-AS2 regulates miR-503/cyclin D1 to promote cell proliferation in non-small cell lung cancer.

Abstract

BACKGROUND:

LncRNA DLGAP1-AS2 plays an oncogenic role in glioma, while its role in other cancers is unknown. This study aimed to study the role of DLGAP1-AS2 in non-small cell lung cancer (NSCLC).

METHODS:

Expression of DLGAP1-AS2 in NSCLC and paired non-tumor tissues from 64 NSCLC patients and the prognostic value of DLGAP1-AS2 for NSCLC were analyzed by performing a 5-year follow-up study. The interaction between DLGAP1-AS2 and miR-503 was confirmed by dual luciferase reporter assay, and their relationship was explored in NSCLC cells transfected with DLGAP1-AS2 expression vector or miR-503 mimic. The roles of DLGAP1-AS2 and miR-503 in regulating cyclin D1 expression were analyzed by RT-qPCR and Western blot. Cell proliferation was analyzed by CCK-8 assay.

RESULTS:

DLGAP1-AS2 was upregulated in NSCLC and predicted poor survival. Interaction between DLGAP1-AS2 and miR-503 was confirmed by dual luciferase activity assay. Overexpression experiments showed that DLGAP1-AS2 and miR-503 overexpression failed to significantly affect the expression of each other. Interestingly, DLGAP1-AS2 overexpression upregulated cyclin D1, a target of miR-503, increased cell proliferation and reduced the effects of miR-503 overexpression on cyclin D1 expression and cell proliferation.

CONCLUSIONS:

DLGAP1-AS2 may regulate miR-503/cyclin D1 to promote cell proliferation in NSCLC.

• Authors: Lu Wang, Lei Tang, Tengfei Ge, Feng Zhu, Dan Liu, Hua Guo, Peng Qian, Ning Xu
• Journal: BMC pulmonary medicine (BMC Pulm Med) Vol. 21 Issue 1 Pg. 277 (Aug 28 2021) ISSN: 1471-2466 [Electronic] England
• PMID: 34454450 (Publication Type: Journal Article)
• Copyright: © 2021. The Author(s).

Chemical References

• CCND1 protein, human
• DLGAP1 protein, human
• MIRN503 microRNA, human
• MicroRNAs
• RNA, Long Noncoding
• SAP90-PSD95 Associated Proteins
• Cyclin D1

Topics

• Aged
• Carcinoma, Non-Small-Cell Lung (genetics, pathology)
• Cell Line, Tumor
• Cell Proliferation (genetics)
• Cyclin D1 (genetics, metabolism)
• Female
• Follow-Up Studies
• Gene Expression Regulation, Neoplastic
• Humans
• Lung Neoplasms (genetics, pathology)
• Male
• MicroRNAs (genetics, metabolism)
• Middle Aged
• Prognosis
• RNA, Long Noncoding (genetics)
• SAP90-PSD95 Associated Proteins (genetics, metabolism)